Fluorescein-Conjugated Goat IgG Fraction To Human Immunoglobulins (IgG, IgA, IgM)

Product is the lyophilized powder of fluorescein-5-isothiocyanate (FITC "Isomer I")-conjugated goat IgG fraction to human immunoglobulins (IgG,IgA,IgM) and buffer salts.

Application Notes

This product is suitable for use as a reagent in immunofluorescence assays (IFA), cell staining (fluorescent microscopy and cell sorting), tissue staining, and blot immunostaining. Note: F(ab')₂ fragments are recommended for staining of cells or tissues which contain Fc receptors. Affinity purified antibodies are recommended to avoid non-specific binding from the inherent antibodies of host animals.

Usage Statement

Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.

Key Applications

Immunoassays | Immunohistochemistry (Paraffin; Acetone-Fixed; 4% PFA Frozen) | Immunofluorescence assays (IFA) | Flow Cytometry (FACS)

SKU	0855156
Antibody Type	Polyclonal Antibody
Application Notes	This product is suitable for use as a reagent in immunofluorescence assays (IFA), cell staining (fluorescent microscopy and cell sorting), tissue staining, and blot immunostaining. Note: F(ab')₂ fragments are recommended for staining of cells or tissues which contain Fc receptors. Affinity purified antibodies are recommended to avoid non-specific binding from the inherent antibodies of host animals.
Base Catalog Number	55156
Concentration	Total Protein: 16.2 mg/mL F/P Ratio: 3.8 mol/mol
Conjugate	FITC
Formulation Details	Lyophilized
Host	Goat
Pack Size	2 mL
Preparation Method	Antibody and FITC are conjugated under defined conditions to obtain the specified molar ratio (3-6mol/mol). Conjugated protein is purified by gel filtration. The product is dialyzed into 0.02M sodiumphosphate, 0.14M sodium chloride, pH 7.3, with 0.05% sodium azide, adjusted to standard titer, filtered through a 0.22 μm filter, vialed, and lyophilized.
Purity	Purified
Solubility	Reconstitution: Reconstitute product with 2 mL of deionized or distilled water. Gentle swirling may be used to speed rehydration. Avoid vigorous shaking of the reconstituted material.
Species Reactivity	Human (IgG, IgA, IgM)
Specificity	This product shows reactivity to human immunoglobulins (IgG;IgA;IgM); cross-reactivity to other species may exist. Antibody activity to non-immunoglobulin serum proteins is not present.
Typical Working Concentration	DILUTION RECCOMENDATIONS: 1:100 - 1:400 for Tissue Staining 1:250 - 1:1,000 for Immuno Blotting 1:1,000 - 1:4,000 for ELISA/FIA.
Usage Statement	Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.