Anti-dog IgG (whole molecule) rabbit IgG fraction, fluorescein-conjugated, 2 mL

Product Description

Product is the lyophilized powder of fluorescein-5-isothiocyanate (FITC "Isomer I")-conjugated rabbit IgG fraction to dog IgG (whole molecule) and buffer salts. Antibody and FITC are conjugated under defined conditions to obtain the specified molar ratio (3-6 mol/mol). Conjugated protein is purified by gel filtration.

Application Notes

This product is suitable for use as a reagent in immunofluorescence assays (IFA), cell staining (fluorescent microscopy and cell sorting), tissue staining, and blot immunostaining.

Usage Statement

Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.

Key Applications

Immunoassays | Immunohistochemistry (Paraffin; Acetone-Fixed; 4% PFA Frozen) | Immunofluorescence assays (IFA) | Flow Cytometry (FACS)

Specifications

SKU	0855326
Alternate Names	Anti-Dog
Antibody Type	Polyclonal Antibody
Application Notes	This product is suitable for use as a reagent in immunofluorescence assays (IFA), cell staining (fluorescent microscopy and cell sorting), tissue staining, and blot immunostaining.
Base Catalog Number	55326
Concentration	Total Protein: 21.9 mg/ml F/P Ratio: 5.95 mol/mol Antibody Protein: 4 mg/ml
Conjugate	FITC
Formulation Details	Lyophilized
Host	Rabbit
Immunogen	Dog IgG
Pack Size	2 mL
Personal Protective Equipment	Eyeshields, Gloves, half-mask respirator, multi-purpose combination respirator cartridge
Preparation Method	Antibody and FITC are conjugated under defined conditions to obtain the specified molar ratio (3-6 mol/mol). Conjugated protein is purified by gel filtration. The product is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, with 0.05% sodium azide, adjusted to standard titer, filtered through a 0.22 µm filter, vialed, and lyophilized.
Purity	Purified
Solubility	Soluble in water
Species Reactivity	Dog (Whole Molecule)
Specificity	This product shows reactivity to dog IgG; cross-reactivity to other species may exist. Because the product is directed against whole IgG; some antibodies are expected to bind light chain sites common to all immunoglobulins. Antibody activity to non-immunoglobulin serum proteins is not present.
Typical Working Concentration	Suggested working dilution: Direct immunofluorescence: 1:32
Usage Statement	Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.