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Product is the lyophilized powder of clarified mouse myeloma ascites IgG2b (j) MOPC 195.
This product is suitable for use as a positive control in antibody identification screening techniques. It gives sharp precipitin lines in immunoelectrophoresis and immunodiffusion techniques. Purified proteins are recommended as ELISA coating antigens and as controls.
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Immunoassays | Immunoblot| Immunohistochemistry
|Alternate Names||Mouse IgG2b/k|
|Antibody Type||Monoclonal Antibody|
|Application Notes||This product is suitable for use as a positive control in antibody identification screening techniques. It gives sharp precipitin lines in immunoelectrophoresis and immunodiffusion techniques. Purified proteins are recommended as ELISA coating antigens and as controls.|
|Base Catalog Number||50344|
|Clone Name||MOPC 195|
|Pack Size||5 mg|
|Personal Protective Equipment||Eyeshields, Gloves, respirator filter|
|Preparation Method||The IgG2b (j) MOPC 195 tumor line is a mineral oil (Bayol F)-induced plasmacytoma. The clarified ascites is produced in BALB/c mice, and is filtered through a 0.22 µm filter, vialed and lyophilized|
|Solubility||Reconstitution: Reconstitute product with 1 mL of deionized or distilled water. Gentle swirling may be used to speed rehydration. Avoid vigorous shaking of the reconstituted material.|
|Specificity||The total protein is measured using the Biuret procedure; with bovine albumin as a standard. Each ascites is tested for immunoglobulin content by radial immunodiffusion with specific antisera following precipitation by ammonium sulfate (See Protocol for Immunoglobulin Precipitation). In addition; the ascites contains the normal threshold of mouse immunoglobulins and ascites proteins.|
|Sterility||Filtered through a .22 µm filter|