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Ascites fluid, collected from Balb/c mice which carry plasmacytomas subcutaneously, are clarified by centrifugation and filtration. It is dialyzed, adjusted to yield at least 5 mg of immunoglobulin, then vialed and lyophilized. Clarified ascites are tested for immunoglobulin by immunoelectrophoresis using antisera specific for the particular type and isotype. Approximately 10% of the total protein is immunoglobulin (including trace levels of the host's immunoglobulins), 80% is albumin, and 10% is complement and other proteins.
This product is suitable for use as a positive control in antibody identification screening techniques and is recommended as an ELISA coating antigen and control.
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
|Antibody Type||Monoclonal Antibody|
|Application Notes||This product is suitable for use as a positive control in antibody identification screening techniques and is recommended as an ELISA coating antigen and control.|
|Base Catalog Number||50341|
|Clone Name||MOPC 21|
|Pack Size||5 mg|
|Solubility||Soluble in Water|
|Specificity||The total protein is measured using the Biuret procedure; with bovine albumin as a standard. Each ascites is tested for immunoglobulin content by radial immunodiffusion with specific antisera following precipitation by ammonium sulfate (See Protocol for mmunoglobulin Precipitation). In addition; the ascites contains the normal threshold of mouse immunoglobulins and ascites proteins|