Anti-mouse IgG (whole molecule) goat IgG fraction, 2 mL

Product Description

Product is the lyophilized powder of goat IgG fraction to mouse IgG (whole molecule) and buffer salts.

Application Notes

Goat IgG fraction to mouse IgG (whole molecule) is used in immunostaining of acetone-fixed frozen and formalin-fixed, paraffin-embedded tissue sections. The antibody was used in ELISA and RIA in various studies. It is also suitable for use as a precipitating antibody. It gives sharp precipitin lines in immunoelectrophoresis and immunodiffusion techniques. This product is suitable for conjugation with enzymes, radiolabels, fluorochromes or other markers, and for attachment to solid supports for use as an immunoadsorbant. This product is suitable for use as a primary reagent in enzyme immunoassays, fluorescein immunoassays and blot, cell or tissue immunostaining. (Note: F(ab')₂ fragments are recommended for staining of cells or tissues which contain Fc receptors. Affinity purified antibodies are recommended to avoid non-specific binding from the inherent antibodies of host animals).

Usage Statement

Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.

Key Applications

Immunoassays | Immunohistochemistry (Paraffin; Acetone-Fixed) | Immunofluorescence assays (IFA)

Specifications

SKU	0855455
Antibody Type	Polyclonal Antibody
Application Notes	Goat IgG fraction to mouse IgG (whole molecule) is used in immunostaining of acetone-fixed frozen and formalin-fixed, paraffin-embedded tissue sections. The antibody was used in ELISA and RIA in various studies. It is also suitable for use as a precipitating antibody. It gives sharp precipitin lines in immunoelectrophoresis and immunodiffusion techniques. This product is suitable for conjugation with enzymes, radiolabels, fluorochromes or other markers, and for attachment to solid supports for use as an immunoadsorbant. This product is suitable for use as a primary reagent in enzyme immunoassays, fluorescein immunoassays and blot, cell or tissue immunostaining. (Note: F(ab')₂ fragments are recommended for staining of cells or tissues which contain Fc receptors. Affinity purified antibodies are recommended to avoid non-specific binding from the inherent antibodies of host animals).
Base Catalog Number	55455
Concentration	Total Protein: 24.6 mg/mL; Antibody Protein: 10 mg/mL
Conjugate	Unconjugated
Formulation Details	Lyophilized
Host	Goat
Immunogen	Pooled normal mouse serum
Pack Size	2 mL
Personal Protective Equipment	Eyeshields, Gloves, Respirator filter
Preparation Method	The IgG fraction is prepared from the specific goat antiserum by delipidation, ammonium sulfate fractionation and anion exchange chromatography. The resulting IgG fraction is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, adjusted to standard titer, filtered through a 0.22 μm filter, vialed and lyophilized.
Solubility	Soluble in water
Species Reactivity	Mouse (Whole Molecule)
Specificity	It shows reactivity to mouse IgG; cross-reactivity to other species may exist. Because the product is directed against whole IgG; some antibodies are expected to bind light chain sites common to all immunoglobulins.
Typical Working Concentration	Anti-Mouse IgG (whole molecule) antibody was used as secondary antibody in immunoblotting at a working dilution of 1:1000.
Usage Statement	Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.