Anti-human Kappa Chain (bound) goat F(Ab')2 fragment, 2 mL
Product is the lyophilized powder of goat F(ab')2 fragment to human kappa chain (bound) and buffer salts.
Application Notes
Goat F(AB')2 fragment to Human (K) chain is use as a precipitating antibody. It gives sharp precipitin lines in immunoelectrophoresis and immunodiffusion techniques. This product is suitable for conjugation with enzymes, radiolabels, fluorochromes or other markers, and for attachment to solid supports for use as an immunoadsorbant. This product is suitable for use as a primary reagent in enzyme immunoassays, fluorescein immunoassays and blot, cell or tissue immunostaining. F(ab')2 fragments are recommended for staining of cells or tissues which contain Fc receptors.
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
Immunoassays | Precipitating antibody |Immunoprecipitation
| SKU | 0855059 |
| Antibody Type | Polyclonal Antibody |
| Application Notes | Goat F(AB')2 fragment to Human (K) chain is use as a precipitating antibody. It gives sharp precipitin lines in immunoelectrophoresis and immunodiffusion techniques. This product is suitable for conjugation with enzymes, radiolabels, fluorochromes or other markers, and for attachment to solid supports for use as an immunoadsorbant. This product is suitable for use as a primary reagent in enzyme immunoassays, fluorescein immunoassays and blot, cell or tissue immunostaining. F(ab')2 fragments are recommended for staining of cells or tissues which contain Fc receptors. |
| Base Catalog Number | 55059 |
| Concentration | Total Protein: 20.4 mg/mL; Antibody Protein: 8 mg/mL |
| Conjugate | Unconjugated |
| Formulation Details | Lyophilized |
| Host | Goat |
| Pack Size | 2 mL |
| Preparation Method | The F(ab')<sub>2</sub> fragment is prepared from the specific goat IgG fraction by limited digestion with pepsin, and purified by gel filtration or affinity chromatography. The resulting F(ab')<sub>2</sub> fragment is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, 0.05% sodium azide, adjusted to standard titer, filtered through a 0.22 μm filter, vialed and lyophilized. |
| Solubility | Reconstitution: Reconstitute product with 2.0 mL of deionized or distilled water. Gentle swirling may be used to speed rehydration. Avoid vigorous shaking of the reconstituted material. |
| Species Reactivity | Human, Kappa Chain (Bound) |
| Specificity | F(ab')2 fragments are tested for purity and specificity at a minimum of 20 mg/ml using immunoelectrophoresis. The product is goat F(ab')2; no trace of albumin; whole molecule IgG or Fc is detected. It shows reactivity to bound kappa light chains; cross-reactivity to other species may exist. Because the product is directed against the kappa chains in Fab; some antibodies are expected to bind light chain sites common to all immunoglobulins. Antibody activity to free kappa light chains may be present. Antibody activity to human lambda Fab; free lambda light chains; or non-immunoglobulin serum proteins is not present. |
| Usage Statement | Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. |
![MP COA [55059] lot ID: 03527](https://www.mpbio.com/static/version20210122092053Z/frontend/Mpbio/default/en_US/images/pdf.png "MP COA [55059] lot ID: 03527")
