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Product is the lyophilized powder of rabbit IgG fraction to human IgG (whole molecule) and buffer salts. The IgG fraction is prepared from the specific rabbit antiserum by delipidation, ammonium sulfate fractionation and anion exchange chromatography. The resulting IgG fraction is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, adjusted to standard titer, filtered through a 0.22 µm filter, vialed and lyophilized.
This product is suitable for use as a precipitating antibody. It gives sharp precipitin lines in immunoelectrophoresis and immunodiffusion techniques.
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
|Antibody Type||Polyclonal Antibody|
|Application Notes||This product is suitable for use as a precipitating antibody. It gives sharp precipitin lines in immunoelectrophoresis and immunodiffusion techniques.|
|Base Catalog Number||55008|
|Pack Size||5 mL|
|Solubility||Soluble in Water|
|Species Reactivity||Human (Whole Molecule)|
|Specificity||The total protein is measured using the Biuret procedure; with bovine albumin as a standard. Antibody titer is standardized to an in-house standard by equivalence-point precipitation. Each IgG fraction is tested for purity and specificity at a minimum of 40 mg/ml using immunoelectrophoresis. The product is mostly rabbit IgG; no trace of albumin is detected. It shows reactivity to human IgG; cross-reactivity to other species may exist. Because the product is directed against whole IgG; some antibodies are expected to bind light chain sites common to all immunoglobulins. Antibody activity to non-immunoglobulin serum proteins is not present.|
|Typical Working Concentration||Reconstitute product with 5.0 ml of deionized or distilled water. Gentle swirling may be used to speed rehydration. Avoid vigorous shaking of the reconstituted material.|