Anti-human IgG (whole molecule) goat F(Ab')2 fragment, 2 mL
Product is the lyophilized powder of goat F(ab')2 fragment to human IgG (whole molecule) and buffer salts.
Application Notes
Product is use as a precipitating antibody. It gives sharp precipitin lines in immunoelectrophoresis and immunodiffusion techniques. It can be used for conjugation with enzymes, radiolabels, fluorochromes or other markers, and for attachment to solid supports for use as an immunoadsorbant. This product is suitable for use as a primary reagent in enzyme immunoassays, fluorescein immunoassays and blot, cell or tissue immunostaining. It is also used in Quantitative Precipitin Assay at concentration of 3.0-4.0 mg/mL in Indirect ELISA(1:80,000).
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
Immunoassays | ELISA | Quantitative precipitin assay
| SKU | 0855049 |
| Antibody Type | Polyclonal Antibody |
| Application Notes | Product is use as a precipitating antibody. It gives sharp precipitin lines in immunoelectrophoresis and immunodiffusion techniques. It can be used for conjugation with enzymes, radiolabels, fluorochromes or other markers, and for attachment to solid supports for use as an immunoadsorbant. This product is suitable for use as a primary reagent in enzyme immunoassays, fluorescein immunoassays and blot, cell or tissue immunostaining. It is also used in Quantitative Precipitin Assay at concentration of 3.0-4.0 mg/mL in Indirect ELISA(1:80,000). |
| Base Catalog Number | 55049 |
| Concentration | Total Protein: 27.2 mg/mL; Antibody Protein: 10 mg/mL |
| Conjugate | Unconjugated |
| Formulation Details | Lyophilized |
| Host | Goat |
| Pack Size | 2 mL |
| Personal Protective Equipment | Eyeshields, Gloves, half-mask respirator, multi-purpose combination respirator cartridge |
| Preparation Method | The F(ab')2 fragment is prepared from the specific goat IgG fraction by limited digestion with pepsin, and purified by gel filtration or affinity chromatography. The resulting F(ab')2 fragment is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, adjusted to standard titer, filtered through a 0.22 µm filter, vialed and lyophilized. No preservative has been added. |
| Solubility | Reconstitution: When reconstituted with 2.0 mL of deionized or distilled water, the product will have an antibody concentration of 10 mg/mL. Gentle swirling may be used to speed rehydration. Avoid vigorous shaking of the reconstituted material. |
| Species Reactivity | Human (Whole Molecule) |
| Specificity | It shows reactivity to human IgG; cross-reactivity to other species may exist. Because the product is directed against whole IgG; some antibodies are expected to bind light chain sites common to all immunoglobulins. |
| Sterility | Filtered through a .22 µm filter |
| Usage Statement | Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. |
![MP COA [55049] lot ID: 00061](https://www.mpbio.com/static/version20201229105009Z/frontend/Mpbio/default/en_US/images/pdf.png "MP COA [55049] lot ID: 00061")
