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Anti-epithelial keratin-AE5 mouse monoclonal antibody , 200 µg

Anti-epithelial keratin-AE5 mouse monoclonal antibody , 200 µg

$492.30

Key features and details

Monoclonal mouse anti-epithelial keratin antibody is specific for the basic 64K keratin which, together with the acidic 55K keratin, has been designated as a marker for "corneal-type differentiation".

SKU: 08691431

Synonyms
Type II Cytokeratin
Packaging size:
200 µg
Product Description

Monoclonal Mouse Anti-Epithelial Keratin antibody is specific for the basic 64K keratin which, together with the acidic 55K keratin, has been designated as a marker for "corneal-type differentiation".

Application Notes

Monoclonal Mouse Anti-Epithelial Keratin - AE5 is used in immunoblotting; Indirect Immunofluorescence; Tissue sections. This antibody is specific for the basic 64 kDa (number 3) keratin which, together with the acidic 55 kDa (number 12) keratin, has been designated as a marker for "corneal-type differentiation." It also reacts with lip and snout (cow and rabbit) epithelia. For this antibody, unfixed, frozen sections perform better than formalin-fixed sections.

Usage Statement

Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.

Key Applications

Immunoassays | Immunoblot | Immunofluorescence assays (IFA) | Immunohistochemistry (Paraffin)

Specifications
SKU 08691431
Alternate Names Type II Cytokeratin
Antibody Type Monoclonal Antibody
Application Notes Monoclonal Mouse Anti-Epithelial Keratin - AE5 is used in immunoblotting; Indirect Immunofluorescence; Tissue sections. This antibody is specific for the basic 64 kDa (number 3) keratin which, together with the acidic 55 kDa (number 12) keratin, has been designated as a marker for "corneal-type differentiation." It also reacts with lip and snout (cow and rabbit) epithelia. For this antibody, unfixed, frozen sections perform better than formalin-fixed sections.
Base Catalog Number 691431
Clone Name AE5
Concentration Antibody Conc.:1.0 mg/ml
Conjugate Unconjugated
Formulation Details Liquid
Host Mouse
Isotype Purified Ig fraction from ascites
Pack Size 200 µg
Preparation Method The AE5 monoclonal anti-keratin was prepared against human epidermal keratin by hybridoma technique using P3 x 63 Ag8 myeloma cells. The AE5 was reactive with the 64K keratins in the cornea.
Solubility Soluble in water
Typical Working Concentration The dilutions below are suggested as staring points. The dilution range for western blotting may be as great as 1:1000 - 1:3000. 1:500 (Immunoblot - human epithelial cornea) 1:50 (Immunofluorescence - human epithelial cornea) It is recommended that each lab obtain their own optimum working dilution.
Usage Statement Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Citations
Documents

Certificates of Analysis

Certificates of Analysis
English (United States)