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Rat liver S9 homogenate in KCL
Rat Liver S9 Homogenate is a Supernatant fraction obtained from an organ (usually liver) homogenate by centrifuging at 9000 g for 20 minutes in a suitable medium; this fraction contains cytosol and microsomes. The microsomes component of the S9 fraction contain cytochrome P450 isoforms (phase I metabolism) and other enzyme activities. The cytosolic portion contains the major part of the activities of transferases (phase II metabolism).
Xanthine oxidase from buttermilk, 1 - 4 U/mg protein
β-Glucosidase from sweet almonds, ≥1000 U/mg protein
Phorbol 12-myristate 13-acetate, molecular biology reagent, ~99%
Phorbol 12-Myristate 13-Acetate
Proteinase K, >30 mAnson U/mg
A highly active stable endopeptidase with a broad spectrum of action was isolated by E. Merk's Darmstadt Biochemical Research Department in 1970 from a culture filtrate of the fungus, Tritirachium album Limber, suitable for both protein and nucleic acid isolation, it exhibits proteolytic activity on proteins, peptides, glycoproteins, amides and esters. Also active with nitroanilides of amino acids with protected amino groups, excluding arginine.
Catalase, 50 KU/mg
Catalase occurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide.It promotes growth of cells including T-cells, B-cells, myeloid leukemia cells, melanoma cells, mastocytoma cells and normal and transformed fibroblast cells.Defects in catalase are the cause of acatalasemia,a metabolic disorder characterized by absence of catalase activity in red cells and is often associated with ulcerating oral lesions.
α-Amylase from porcine pancreas, ≥700 U/mg protein