Ammonium Sulfate
Application Notes
Ammonium sulfate is a widely used reagent in molecular biology and chromatography. It is useful in the isolation and purification of enzymes and proteins. It is used for the precipitation or fractionation of proteins or for purification of antibodies.It is also useful for crystallographic analysis of nucleic acids and proteins. Ammonium sulfate is also widely used in HPLC of proteins, such as in hydrophobic interaction chromatography. Ammonium sulfate is used in long PCR buffer, in PCR lysis solution, and in secondstrand cDNA synthesis. Also it is used for the HPLC analysis of phospholipids.
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
Isolation and purification of proteins
| SKU | 04808211-CF |
| Alternate Names | Sulfuric acid diammonium salt; Mascagnite |
| Application Notes | Ammonium sulfate is a widely used reagent in molecular biology and chromatography. It is useful in the isolation and purification of enzymes and proteins. It is used for the precipitation or fractionation of proteins or for purification of antibodies.It is also useful for crystallographic analysis of nucleic acids and proteins. Ammonium sulfate is also widely used in HPLC of proteins, such as in hydrophobic interaction chromatography. Ammonium sulfate is used in long PCR buffer, in PCR lysis solution, and in secondstrand cDNA synthesis. Also it is used for the HPLC analysis of phospholipids. |
| Auto Ignition | Not flammable (USCG, 1999) |
| Base Catalog Number | 808211 |
| CAS # | 7783-20-2 |
| Density | 1.78 at 59° F (USCG, 1999) |
| EC Number | 231-984-1 |
| Format | Crystalline powder |
| Grade | Ultra Pure |
| Melting Point | 280 deg C (decomposes) |
| Molecular Formula | (NH4)2SO4 |
| Molecular Weight | 132.134 g/mol |
| Personal Protective Equipment | Eyeshields, Gloves, respirator filter |
| pH | pH = 5.5 (0.1 M aqueous solution) |
| Purity | ≥99% |
| RTECS Number | BS4500000 |
| Solubility | Freely soluble in <a class="pubchem-internal-link CID-962" href="https://pubchem.ncbi.nlm.nih.gov/compound/water">water</a>, insoluble in <a class="pubchem-internal-link CID-702" href="https://pubchem.ncbi.nlm.nih.gov/compound/ethanol">ethanol</a> |
| Usage Statement | Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. |
| UV Visible Absorbance | OD260 (saturated soln) : ≤0.1 , OD280 (saturated soln) : ≤0.1 |
| Vapor Pressure | Vapor pressure of saturated solution: 0.901 kPa at 10 deg C; 1.319 kPa at 15 deg C; 1.871 kPa at 20 deg C; 2.573 kPa at 25 deg C; 3.439 kPa at 30 deg C; 4.474 kPa at 35 deg C |
![MP COA [04808211-CF] lot ID: YC0810](https://www.mpbio.com/static/version1741337905/frontend/Mpbio/default/en_US/images/document.png "MP COA [04808211-CF] lot ID: YC0810")