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2-Mercaptoethanol, electrophoresis grade, 25 g

2-Mercaptoethanol, electrophoresis grade, 25 g

Synonyms β-Mercaptoethanol; 2-Hydroxyethylmercaptan; BME; Thioethylene glycol; 2-Hydroxy-1-ethanethiol; Monothioethyleneglycol

CAS Number: 60-24-2 Molecular Formula: C2H6OS Molecular Weight: 78.13

Beilstein Registry Number: 773648 EC Number: 200-464-6 MDL Number: MFCD00004890

Packaging size: 25 g

SKU 04806443
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SKU 04806443
Alternate Names β-Mercaptoethanol; 2-Hydroxyethylmercaptan; BME; Thioethylene glycol; 2-Hydroxy-1-ethanethiol; Monothioethyleneglycol
Application Notes 2-Mercaptoethanol is used to reduce disulfide linkages in solubilizing proteins for gel electrophoresis (typically used in SDS-PAGE sample buffer at 5% concentration). Also reduces excess oxidative polymerization of catalysts. Cleaving intermolecular (between subunits) disulfide bonds allows the subunits of a protein to separate independently on SDS-PAGE. Cleaving intramolecular (within subunit) disulfide bonds allows the subunits to become completely denatured so that each peptide migrates according to its chain length with no influence due to secondary structure. In solution, 2-mercaptoethanol is readily oxidized in air to a disulfide, especially at alkaline pH. Because of this property, it is widely used to protect proteins, enzymes in particular, from becoming inactive. An excess of 2-mercaptoethanol (generally used at 0.01 M) will maintain the protein thiol groups in their reduced state.
Base Catalog Number 806443
Beilstein Registry Number 773648
Boiling Point 157 °C (Lit.)
CAS # 60-24-2
Density 1.114 g/mL at 25 °C (Lit.)
EC Number 200-464-6
Flash Point 68 °C/154.4 °F (Lit.)
Grade Electrophoresis grade
Melting Point <-50 °C (Lit.)
Molecular Formula C2H6OS
Molecular Weight 78.13
Pack Size 25 g
Personal Protective Equipment Faceshields, full-face respirator, Gloves, Goggles, multi-purpose combination respirator cartridge, respirator filter
pH 5.0 (0.1M in water)(Lit.)
Purity ≥98%
RTECS Number KL5600000
Safety Symbol GHS05, GHS06, GHS08, GHS09
Solubility Miscible with water, alcohol, ether and benzene. Solutions containing even trace levels (nanomolar) of some metal salts (especially copper (II) or cobalt(II)) are unstable at pH > 5 and progressively less stable at higher pH values. Most buffers (especially phosphate) contain enough metal salts to lead to substantial loss of -SH within 1-2 days. The addition of EDTA helps to stabilize solutions. If diluted product needs to be stored it is recommended to store at pH 6-7 with EDTA (0.05 mM) at 2-8 °C for no more than 2 to 3 days; however, we highly recommend adding neat 2-mercaptoethanol as needed to the sample.
Usage Statement Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Vapor Density 2.69 (vs air)(Lit.)
Vapor Pressure 1 mmHg (20 °C)(Lit.)