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M9 minimal medium salts, 450 g

M9 minimal medium salts, 450 g

Synonyms M9 Minimal Media

Packaging size: 454 g

SKU 113035022
$133.65
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Description

Product Description

M9 Minimal Salts are a commonly used base for preparing a defined synthetic medium for the growth of most E. coli host cells. The powder is reconstituted in sterile purified water, (or water with melted purified agar added.) Additional nutrients, such as glucose or other carbon source, casamino acids, vitamins, or antibiotics are added when the solution cools and before plates are poured or media is inoculated with bacteria.

Application Notes

M9 Minimal medium salts are suitable for the preparation of M9 Minimal Medium for use in propagation of E. coli and plasmid amplification. The resulting M9 medium can be used for maintaining positive selection pressure on plasmids coding for genes to produce essential substances such as amino acids and vitamins and for maintenance of F’-containing bacteria strains for M13 propagation.

Usage Statement

Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.

Key Applications

Cell culture |Proteomics |Purification and Detection |Recombinant Protein Expression and Analysis |Bacterial Culture |Plasmid Production |Protein Expression |Gene Expression |Cloning |Genetic Manipulation |Growth and Maintenance of M13 phage & other filamentous ssDNA bacteriophages

Specifications
SKU 113035022
Alternate Names M9 Minimal Media
Application Notes M9 Minimal medium salts are suitable for the preparation of M9 Minimal Medium for use in propagation of E. coli and plasmid amplification. The resulting M9 medium can be used for maintaining positive selection pressure on plasmids coding for genes to produce essential substances such as amino acids and vitamins and for maintenance of F’-containing bacteria strains for M13 propagation.
Base Catalog Number 3035022
Components Part A (g/L of 50x): 350 g K2HPO4 100 g KH2HPO4
Part B (g/L of 50x): 29.4 g sodium citrate, 50 g (NH4)2SO4 , 5g MgSO4
Format Powder
Formulation Details Part A (g/L of 50x): 350 g K2HPO4; 100 g KH2HPO4 Part B (g/L of 50x): 29;4g sodium citrate; 50g (NH4)2SO4; 5 g MgSO4
Grade Molecular Biology Certified™
Media Type M9 Minimal Medium
Pack Size 454 g
Personal Protective Equipment Personal Protective Equipment Respiratory protection: Breathing apparatus only if aerosol or dust is formed. Hand protection: Pvc or other plastic material gloves Skin and body protection: Usual safety precautions while handling the product will provide adequate protection against this potential effect. Eye protection: Safety glasses with side-shields Hygiene measures: Handle in accordance with good industrial hygiene and safety practice.
pH Acceptable pH range is: 7.2
Preparation Method Media Preparation:Reagents are tested and chosen for their ability to promote optimum growth of E. coli when combined in M9 Minimal Medium Salt formulations. Chosen reagents are combined in the proper proportions according to the formulation listed in the Certificate of Analysis and thoroughly blended for a uniform distribution.
Solubility Soluble in water
Sterilization of Solutions A 45 g sample of M9 Minimal Salts Part A was dissolved in water to 100 mL, the solution was autoclaved to make a sterile 50 X concentrate. A 8.4 g sample of M9 Minimal Salts Part B was dissolved in water to 10 mL, the solution was autoclaved to make a sterile 50 X concentrate.
Usage Statement Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.