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RPM Yeast Plasmid Isolation Kit, 100 preps

RPM Yeast Plasmid Isolation Kit, 100 preps

Synonyms Yeast Plasmid Miniprep Purification Kit

Packaging size: 100 Preps

SKU 112069400
$457.00
In Stock
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Description

Product Description

The RPM™ Yeast Plasmid Isolation Kit allows isolation of plasmid DNA from yeast cells. It uses a GLASSMILK purification process in conjunction with SPIN modules to increase yield and achieve optimal DNA quality. The isolated DNA can be used for screening and bacterial transformations.

Application Notes

The Yeast Plasmid Isolation Kit is a Rapid Pure Miniprep Kit designed for yeast cells. The plasmid isolated from yeast is suitable for PCR and bacterial transformation. The same kit can be used to isolate plasmid from bacterial transformants for use in any downstream application (PCR, sequencing, restriction enzyme digestion, labeling, etc). Plasmid DNA mini-preps from yeast. Rescue positive prey plasmids after a yeast two-hybrid or a yeast one-hybrid screen.

Usage Statement

Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.

Key Applications

Isolation of yeast plasmid DNA

Specifications
SKU 112069400
Alternate Names Yeast Plasmid Miniprep Purification Kit
Application Notes The Yeast Plasmid Isolation Kit is a Rapid Pure Miniprep Kit designed for yeast cells. The plasmid isolated from yeast is suitable for PCR and bacterial transformation. The same kit can be used to isolate plasmid from bacterial transformants for use in any downstream application (PCR, sequencing, restriction enzyme digestion, labeling, etc). Plasmid DNA mini-preps from yeast. Rescue positive prey plasmids after a yeast two-hybrid or a yeast one-hybrid screen.
Base Catalog Number 2069400
Components Yeast Lysing Matrix |Alkaline Lysis Solution |Neutralizing Solution |GLASSMILK™ Spin Buffer |Wash Solution Concentrate |SPIN Filters |Catch Tubes |Pre-Lysis Buffer
Format Geneclean® GLASSMILK™ slurry plus SPIN filter
Pack Size 100 Preps
Personal Protective Equipment Personal-protective-equipment: Hand protection: Latex, Nitrile or other plastic material gloves. Skin and body protection: Labcoat and sleves. Eye protection: Safety glasses with side-shields Hygiene measures: Handle in accordance with good industrial hygiene and safety practice.
pH Alkaline Lysis Solution pH Range: 12.6-12.8 |Neutralizing Solution pH Range: 5.5-5.9 |Wash Solution Concentrate pH Range: 7.4-7.7 |Pre-Lysis Buffer pH Range: 6.6-7.0
Preparation Method Before first use, add the 56 mL of 200 proof (100% ethanol) to the Wash Solution Concentrate (56 mL) and mix well. Do not use denatured alcohol because it can cause precipitation of salts. Label the container. Store tightly capped at 15°-30°C.
Safety Symbol GHS07 |GHS05
Sample Source Yeast and Fungi
Sample Type Yeast Plasmid Prep
Sample Volume 1.5 ml of yeast culture
Unit Definition Yields up to 10 μg of plasmid DNA Per 1.5 ml overnight culture typical
Usage Statement Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.