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Rabbit peroxidase anti-peroxidase (PAP), 5 mg
Product is the lyophilized powder of rabbit peroxidase anti-peroxidase complexes (PAP) and buffer salts
Rabbit Peroxidase Anti-Peroxidase Soluble Complex is suitable for use in peroxidase enzymatic labeling experiments, intended for use in immunostaining of cells, tissues, and blots. It is also suitable for use in ELISA and other enzyme immunoassays.
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Immunoassays | Peroxidase enzymatic labeling experiments | Immunohistochemistry
|Antibody Type||Polyclonal Antibody|
|Application Notes||Rabbit Peroxidase Anti-Peroxidase Soluble Complex is suitable for use in peroxidase enzymatic labeling experiments, intended for use in immunostaining of cells, tissues, and blots. It is also suitable for use in ELISA and other enzyme immunoassays.|
|Base Catalog Number||55968|
|Pack Size||5 mg|
|Preparation Method||Prepared by the method of Stemberger. Antibodies to peroxidase are purified by immunoprecipitation with purified horseradish peroxidase (Rz>3.0) at the equivalence point. After extensive washing, the immunoprecipitate is solubilized at low pH. PAP complexes are made soluble by addition of excess peroxidase and neutralization and are purified from free peroxidase by repeated salt fractionation. The resulting PAP complexes are dialyzed into 0.01M sodium phosphate, 0.25M sodium chloride, pH 7.6, adjusted to standard protein concentration, filtered through a 0.22 μm filter, vialed and lyophilized. The product may contain preservative.|
|Solubility||Soluble in water|