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Anti-dog IgG (whole molecule) rabbit IgG fraction, fluorescein-conjugated, 2 mL
Product is the lyophilized powder of fluorescein-5-isothiocyanate (FITC "Isomer I")-conjugated rabbit IgG fraction to dog IgG (whole molecule) and buffer salts.
FITC-Conjugated Goat IgG Fraction to Anti-Human Complement C4 is used as a reagent in immunofluorescence assays (IFA), cell staining (fluorescent microscopy and cell sorting), tissue staining, and blot immunostaining.
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Immunoassays | Immunohistochemistry (Paraffin; Acetone-Fixed; 4% PFA Frozen) | Immunofluorescence assays (IFA) | Flow Cytometry (FACS)
|Antibody Type||Polyclonal Antibody|
|Application Notes||FITC-Conjugated Goat IgG Fraction to Anti-Human Complement C4 is used as a reagent in immunofluorescence assays (IFA), cell staining (fluorescent microscopy and cell sorting), tissue staining, and blot immunostaining.|
|Base Catalog Number||55326|
|Concentration||Total Protein: 21.9 mg/ml F/P Ratio: 5.95 mol/mol Antibody Protein: 4 mg/ml|
|Pack Size||2 mL|
|Personal Protective Equipment||Eyeshields, Gloves, half-mask respirator, multi-purpose combination respirator cartridge|
|Preparation Method||Antibody and FITC are conjugated under defined conditions to obtain the specified molar ratio (3-6 mol/mol). Conjugated protein is purified by gel filtration. The product is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, with 0.05% sodium azide, adjusted to standard titer, filtered through a 0.22 µm filter, vialed, and lyophilized.|
|Solubility||Soluble in water|
|Species Reactivity||Dog (Whole Molecule)|
|Specificity||This product shows reactivity to dog IgG; cross-reactivity to other species may exist. Because the product is directed against whole IgG; some antibodies are expected to bind light chain sites common to all immunoglobulins. Antibody activity to non-immunoglobulin serum proteins is not present.|
|Typical Working Concentration||Suggested working dilution: Direct immunofluorescence: 1:32|