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Anti-human Fibronectin goat IgG fraction, peroxidase-conjugated , 2 mL
Product is the lyophilized powder of horseradish peroxidase (HRP)-conjugated goat IgG fraction to human fibronectin and buffer salts.
Peroxidase-Conjugated Goat IgG Fraction to Human Fibronectin is suitable for use as a reagent in enzyme immunoassays (EIA), cell and tissue staining (for light microscopy), cell and tissue labeling (for electron microscopy), and blot immunostaining. (Note: F(ab')2 fragments are recommended for staining of cells or tissues which contain Fc receptors).
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Immunoassays | ELISA | Immunoblot | Immunohistochemistry | EM-Immunohistochemistry
|Antibody Type||Polyclonal Antibody|
|Application Notes||Peroxidase-Conjugated Goat IgG Fraction to Human Fibronectin is suitable for use as a reagent in enzyme immunoassays (EIA), cell and tissue staining (for light microscopy), cell and tissue labeling (for electron microscopy), and blot immunostaining. (Note: F(ab')2 fragments are recommended for staining of cells or tissues which contain Fc receptors).|
|Base Catalog Number||55240|
|Concentration||Total Protein: 63.2 mg/mL; Titer: 1:38;800|
|Pack Size||2 mL|
|Preparation Method||Antibody and highly purified HRP (Rz>3.0) are conjugated under defined conditions to obtain optimally labeled product. Conjugated protein is purified by salt fractionation. The product is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, with 0.01% thimerosal, adjusted to standard titer, filtered through a 0.22 µm filter, vialed and lyophilized.|
|Solubility||Soluble in water|
|Specificity||It shows reactivity to human fibronectin; cross-reactivity to other species may exist. Antibody activity to human fibrinogen or other serum proteins is not present.|
|Typical Working Concentration||Dilution Recommended: 1:500 - 1:4,000 for Tissue Staining; 1:1,000 - 1:8,000 for Immuno Blotting; 1:2,000 - 1:16,000 for ELISA/FIA|