IgG2A (Kappa) UPC 10, mouse myeloma, purified, 1 mg

Description

Product Description

Product is purified mouse IgG2a (Kappa) and buffer. Mouse IgG2a (Kappa) is purified from clarified mouse UPC 10 ascites using multi-step procedures which may include salt fractionation, gel filtration, ion-exchange chromatography, affinity chromatography and immunoabsorption. The product is dialyzed into 0.02M TRIS, 0.15M sodium chloride, pH 8.1, filtered through a 0.22 µm filter and vialed.

Application Notes

This product is suitable for use as a positive control in antibody identification screening techniques and is recommended as an ELISA coating antigen and control.

Usage Statement

Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.

Key Applications

Immunoassays | ELISA | Control

Specifications

SKU	0850328
Alternate Names	Mouse IgG2a/k
Antibody Type	Monoclonal Antibody
Application Notes	This product is suitable for use as a positive control in antibody identification screening techniques and is recommended as an ELISA coating antigen and control.
Base Catalog Number	50328
Clone Name	UPC 10
Concentration	Total Protein: 5.0 mg/mL
Conjugate	Unconjugated
Formulation Details	Purified
Host	Mouse
Isotype	IgG
Pack Size	1 mg
Personal Protective Equipment	Eyeshields, Gloves, respirator filter
Preparation Method	Mouse IgG2a (j) is purified from clarified mouse UPC 10 ascites using multi-step procedures which may include salt fractionation, gel filtration, ion-exchange chromatography, affinity chromatography and immunoabsorption. The product is dialyzed into 0.01M Sodium Phosphate, 0.13M sodium chloride, pH 7.2, and vialed.
Purity	Affinity Purified
Solubility	Soluble in Water
Specificity	The total protein is determined by absorbance at 280nm. The product contains 1 mg of mouse IgG2a. The product is tested for purity at 1.0 mg/ml using immunoelectrophoresis. The product is mouse IgG2a; no trace of IgG1; IgG2b; IgG3; IgA; IgM or non-immunoglobulin serum proteins is detected.
Sterility	Filtered through a .22 µm filter
Usage Statement	Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.