IgG2A (Kappa) UPC 10, mouse myeloma, purified, 1 mg
Product is purified mouse IgG2a (Kappa) and buffer. Mouse IgG2a (Kappa) is purified from clarified mouse UPC 10 ascites using multi-step procedures which may include salt fractionation, gel filtration, ion-exchange chromatography, affinity chromatography and immunoabsorption. The product is dialyzed into 0.02M TRIS, 0.15M sodium chloride, pH 8.1, filtered through a 0.22 µm filter and vialed.
Application Notes
This product is suitable for use as a positive control in antibody identification screening techniques and is recommended as an ELISA coating antigen and control.
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
Immunoassays | ELISA | Control
| SKU | 0850328 |
| Alternate Names | Mouse IgG2a/k |
| Antibody Type | Monoclonal Antibody |
| Application Notes | This product is suitable for use as a positive control in antibody identification screening techniques and is recommended as an ELISA coating antigen and control. |
| Base Catalog Number | 50328 |
| Clone Name | UPC 10 |
| Concentration | Total Protein: 5.0 mg/mL |
| Conjugate | Unconjugated |
| Formulation Details | Purified |
| Host | Mouse |
| Isotype | IgG |
| Pack Size | 1 mg |
| Personal Protective Equipment | Eyeshields, Gloves, respirator filter |
| Preparation Method | Mouse IgG2a (j) is purified from clarified mouse UPC 10 ascites using multi-step procedures which may include salt fractionation, gel filtration, ion-exchange chromatography, affinity chromatography and immunoabsorption. The product is dialyzed into 0.01M Sodium Phosphate, 0.13M sodium chloride, pH 7.2, and vialed. |
| Purity | Affinity Purified |
| Solubility | Soluble in Water |
| Specificity | The total protein is determined by absorbance at 280nm. The product contains 1 mg of mouse IgG2a. The product is tested for purity at 1.0 mg/ml using immunoelectrophoresis. The product is mouse IgG2a; no trace of IgG1; IgG2b; IgG3; IgA; IgM or non-immunoglobulin serum proteins is detected. |
| Sterility | Filtered through a .22 µm filter |
| Usage Statement | Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. |
![MP COA [50328] lot ID: 00463](https://www.mpbio.com/static/version20201229105009Z/frontend/Mpbio/default/en_US/images/pdf.png "MP COA [50328] lot ID: 00463")
