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Isotype: mouse IgG1
Applications: Immunoblotting; Immunostaining of cell suspensions, acetone-fixed frozen tissue sections, and formalin-fixed, paraffin-embedded (microwaved) tissue sections.
The original antigen was purified calf lens. The antibody reacts specifically to 57 kDa vimentin protein in immunoblotting. Additionally, it reacts specifically with cells of mesenchymal origin including fibroblasts, endothelial cells, smooth muscle cells, chondrocytes, lymphocytes and blood cells. It cross-reacts with vimentin of most mammals including human, mouse, rat and rabbit. It is supplied as clarified cell culture supernatant with 1% BSA and 0.09% sodium azide.
Ref.: Van Muijen, G.N., et al., Lab. Invest., 57(4): 359-369, (1987).
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|Antibody Type||Monoclonal Antibody|
|Application Notes||Immunoblotting; Immunostaining|
|Base Catalog Number||10515|
|Immunogen||Isolated from Calf lens|
|Pack Size||1 mL|
|Solubility||Soluble in Water|
|Specificity||Immunoblotting: Antibody reacts with the 57 kD vimentin protein. Cross-reactivity with Vimentin in most mammals.|
|Typical Working Concentration||Dilution: 1:10|