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T4 Bacteriophage Gene 32 protein (T4 gp 32)

T4 Bacteriophage Gene 32 protein (T4 gp 32)
£973.00

Key features and details

T4 bacteriophage gene 32 protein (T4 gp 32) is a specific single-strand-binding protein (purified recombinant form) from the T4 bacteriophage. It is reported to act by binding to single-stranded DNA in a cooperative manner thereby destabilizing double-stranded DNA.

SKU: 11TGP32500

Packaging size:
500 µg
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Product Description

T4 gp32 is used to improve:

  • Yields of long PCR products catalyzed by Taq DNA Polymerase
  • Amplification efficiency in low purity DNA
  • DNA Polymerase proofreading
  • Restriction enzyme digestion
  • DNA sequencing using the Sanger method
  • Stabilizing of DNA single-stranded regions for site-directed mutagenesis

Product Includes:

  • T4 gp32 Protein
  • 10x T4 gp32 Reaction Buffer
  • 1x T4 gp32 Dilution Buffer

1x T4gp 32 Reaction Buffer Composition: 20 mM Tris-acetate, 100 mM Potassium acetate, 10 mM Magnesium acetate, 1 mM DTT, pH 7.8 @ 25ºC

1x T4gp 32 Dilution Buffer Composition: 50 mM Tris-HCl, 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol, pH 7.5 @ 25ºC


Product Source 

E. Coli BL21 (DE3) strain expressing T4 gp32 gene.

Heat Inactivation

65°C for 20 min

 

Quality Control Assays

T4 gp32 protein is free from detectable nuclease activities.

Specifications
SKU 11TGP32500
Pack Size 500 µg
Storage -20°C
Documents

Datasheet

Datasheet
English (United States)

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