SPINeasy Plasmid Midiprep Kit is a silica-membrane spin-column kit for the purification of high-copy and low-copy number plasmid DNA from 25 – 50 mL of transformed bacterial culture. Using our specially formulated buffers, bacterial cells are disrupted by alkaline lysis to release plasmid DNA. Cell debris, such as chromosomal DNA and proteins, are precipitated by neutralization and cleared through centrifugation. Subsequently, plasmid DNA in the lysate supernatant is adsorbed on a silica membrane, washed and eluted in a convenient process using the spin column technology. Up to 1 mg of plasmid DNA can be purified through this simple workflow, which is immediately ready for routine molecular biology laboratory applications.
Kit performance
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Figure 1: Agarose gel electrophoresis result of pcDNA3.1 plasmid DNA extracted using the SPINeasy Plasmid Midiprep Kit with downstream molecular applications. M: DNA marker; Lane 1: Purified plasmid DNA; Lane 2: Plasmid digested with two single cut restriction enzymes, yielding two bands; Lane 3: PCR of plasmid, producing a band of the expected amplicon size; Lane 4: PCR negative control. |
Application Notes
The SPINeasy Plasmid Midiprep Kit is a silica membrane spin column kit for the purification of high-copy and low-copy number plasmid DNA from 25 – 50 mL of transformed bacterial cultures.
Usage Statement
Research Use Only (RUO).
Key Applications
In-vitro transcription, cloning, NGS, PCR, qPCR, restriction digestion, transformation, Genomics, Molecular Biology
| SKU | 116539025 |
| Alternate Names | SPINeasy Plasmid Midiprep Extraction Kit; Plasmid DNA Purification |
| Application Notes | The SPINeasy Plasmid Midiprep Kit is a silica membrane spin column kit for the purification of high-copy and low-copy number plasmid DNA from 25 – 50 mL of transformed bacterial cultures. |
| Base Catalog Number | 65390 |
| Product Line | SPINeasy |
| Components | 60 mL Resuspension Buffer SD1, 60 mL Alkaline Lysis Buffer SD2, 90 mL Neutralization Buffer SD3, 70 mL Wash Buffer DW1, 12 mL Wash Buffer DW2, 30 mL Elution Buffer SD, 600 µL RNase A, 25x Column SD with collection tubes |
| Hazard Statements | Hazard Class 8 |
| Pack Size | 25 Preps |
| Personal Protective Equipment | Safety Goggles/Faceshield; Gloves |
| Sample Type | Bacteria (Gram +/-) |
| Sample Volume | 25 - 50 mL bacterial (E. coli) cultures |
| Storage | Store at Room Temperature (15-30℃), Dry environment |
| Usage Statement | Research Use Only (RUO). |
