Freund's Complete Adjuvant

This product is made by a modification of Freund's technique, in which of heat-killed Mycobacterium tuberculosis (0.05%) is suspended in a mixture of paraffin oil (85%) and Arlacel A (15%). Freund's Complete Adjuvant has been widely used as a potent adjuvant in immunization protocols and vaccine development.

The Mycobacterium tuberculosis in Freund's Complete Adjuvant contains a unique glycolipid trehalose 6,6’ dimycolate (TDM, also known as cord factor), which stimulates macrophage-inducible C-type lectin (Mincle). Additionally, FCA has ligands for TLR2, TLR4, and TLR9.

The oil emulsion in Freund's Complete Adjuvant helps to retain the antigen at the injection site, providing a slow release of the antigen over an extended period, thus facilitating a prolonged and robust immune response. The oil emulsion also triggers the activation of complement and other effector molecules that enhance the clearance of the antigen and the recruitment of immune cells to the injection site.

Freund's Complete Adjuvant is primarily used as an initial injection in immunization protocols, as subsequent injections with Freund's Complete Adjuvant are generally avoided due to the risk of inducing adverse reactions. To minimize side-effects, Freund’s Incomplete Adjuvant (SKU:08642861-CF MP Biomedicals) is used for the boosts. Freund's Complete Adjuvant has been used to enhance the immune response to a wide range of antigens, including proteins, peptides, nucleic acids, and carbohydrates.

Application Notes

• Freund's Complete Adjuvant is commonly used in the production of water-in-oil emulsions of immunogens, particularly for antibody production and vaccine development.
• The Mycobacterium tuberculosis in Freund's Complete Adjuvant can activate immune cells leading to the production of cytokines and chemokines that promote inflammation and antigen presentation.
• Freund's Complete Adjuvant enhances the immune response to compounds that are already immunogenic; they do not confer immunogenicity to non-immunogenic haptens.
• Improper use of Freund’s adjuvant can cause undesirable and painful side effects such as excessive inflammation and swelling, abscess formation, ulceration, and tissue necrosis.
• Freund's Complete Adjuvant is typically used only for initial injections, as subsequent injections with Freund's Complete Adjuvant can cause granuloma formation and other complications.

Usage Statement

Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.

Key Applications

Freund's Complete Adjuvant is a potent adjuvant that plays a crucial role in enhancing the immune response to immunogens by promoting sustained release of the antigen and activating immune cells at the injection site. This adjuvant has wide-ranging applications in immunization protocols and vaccine development, which have significantly contributed to the development of effective vaccines and immunotherapies.