SPINeasy PCR Purification and Gel Extraction Kit is a silica-membrane spin-column kit that enables quick and convenient DNA clean up from various enzymatic reactions, such as PCR and restriction digestion, as well as isolation and purification of DNA fragments from agarose gel electrophoresis. Up to 23 µg of DNA of molecular weight ranging from 100 bp to 20 kb can be purified through a quick and simple process. Purified DNA is immediately ready for routine molecular biology laboratory applications.
Kit performance
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Figure 1: Agarose gel electrophoresis of PCR purified DNA using SPINeasy PCR Purification and Gel Extraction Kit. M: DNA marker; Lane 1: Unpurified PCR reaction (input); Lane 2: Purified PCR product; Lane 3: Unpurified plasmid restriction digest reaction (input); Lane 4: Purified restriction digested plasmid. |
| Figure 2: Agarose gel electrophoresis of gel extracted DNA using SPINeasy PCR Purification and Gel Extraction Kit. M: DNA marker; Lane 1: Unpurified PCR reaction (input); Lane 2: Gel extracted PCR product; Lane 3: Unpurified plasmid restriction digest reaction (input); Lane 4: Restriction digested plasmid purified by gel extraction. |  |
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Application Notes
SPINeasy PCR Purification and Gel Extraction Kit is a silica-membrane spin-column kit that enables quick and convenient DNA clean up from various enzymatic reactions, such as PCR and restriction digestion, as well as isolation and purification of DNA fragments from agarose gel electrophoresis.
Usage Statement
Research Use Only (RUO)
Key Applications
DNA clean-up; Primer, nucleotide, enzyme, agarose, salt removal from PCR, restriction digestion reactions, and from agarose gels for purified DNA, ready for downstream applications including cloning, NGS library preparation, in-vitro transcription, Genomics, Molecular Biology
| SKU | 116538050 |
| Alternate Names | SPINeasy PCR Purification & Gel Extraction Kit; PCR and Enzymatic Clean-up Kit; DNA Clean-up Kit. |
| Application Notes | SPINeasy PCR Purification and Gel Extraction Kit is a silica-membrane spin-column kit that enables quick and convenient DNA clean up from various enzymatic reactions, such as PCR and restriction digestion, as well as isolation and purification of DNA fragments from agarose gel electrophoresis. |
| Base Catalog Number | 65380 |
| Product Line | MP |
| Components | 50 mL Binding Buffer PG, 6 mL Wash Buffer PG, 10 mL Elution Buffer PG, 50x Column PG with collection tubes |
| Hazard Statements | Hazard Class 8 |
| Pack Size | 50 Preps |
| Personal Protective Equipment | Safety Goggles/Faceshield; Gloves |
| Storage | Store at Room Temperature (15-30 °C), in a dry environment |
| Usage Statement | Research Use Only (RUO) |
![MP COA [116538050] lot ID: FD02004](https://www.mpbio.com/static/version1747301781/frontend/Mpbio/default/en_US/images/document.png "MP COA [116538050] lot ID: FD02004")
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