The SPINeasy DNA Purification Kit is designed for fast and efficient cleanup of pre-purified DNA samples contaminated with a wide range of inhibitory compounds such as those found in environmental sample (humic acid, heavy metals…) as well as chemical compounds which might originate from ineffective purification procedures. Since problematic samples may contain diverse amounts of humic acid contaminant, the SPINeasy DNA Purification Kit provides two choices of binding buffers. The Buffer P1 is recommended for purification of samples with slight contamination of humic acids (light brownish color) or other PCR inhibitors. While the Buffer P1HA contains a novel and proprietary humic acid removal technology, which is recommended to process samples contaminated with considerably amount of humic substances (strong brownish color).
This kit has been validated with genomic DNA isolated from various problematic soils as well as with DNA samples spiked with humic acid molecules. The data shows that the kit performs well on any DNA sample that contains >200bp. Isolated samples can be readily used in downstream applications, including long fragment PCR, qPCR, and next-generation sequencing (16S and whole genome).
Performance :-
A. SPINeasy DNA Purification Kit improves A260/280 and A260/230 ratios of sample contaminated with chemicals (SDS, chaotropic salts, solvents, humic acid) or both chemicals and proteins.
B. DNA samples spiked with humic acid were purified using SPINeasy DNA Purification Kit or a competitor Z kit. Using end point PCR (left), no amplification was observed in absence of purification as well as after purification with competitor Z kit due to the presence of inhibitory humic acid. Conversely, all the PCR products were successfully obtained upon purification with SPINeasy DNA Purification Kit, indicating an efficient removal of humic acid. In line with this, as compared to SPINeasy DNA Purification Kit, a delayed quantitative amplification was observed with competitor Z(quantitative PCR, right). Endpoint and quantitative PCR were performed in a final volume of 20 μL, using ~120 ng and ~50 ng of DNA, respectively.
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Application Notes
The SPINeasy DNA Purification Kit is designed for fast and efficient cleanup of pre-purified DNA samples contaminated with a wide range of inhibitors and chemical compounds.
Usage Statement
Research Use Only (RUO)
Key Applications
DNA clean-up, Inhibitor removal, restriction digests, PCR, qPCR, NGS
| SKU | 116548050 |
| Alternate Names | SPINeasy DNA Clean-up Kit, PCR Inhibitor Removal Kit, DNA Clean-up |
| Application Notes | The SPINeasy DNA Purification Kit is designed for fast and efficient cleanup of pre-purified DNA samples contaminated with a wide range of inhibitors and chemical compounds. |
| Base Catalog Number | 65480 |
| Product Line | SPINeasy |
| Components | Buffer P1 50 mL, Buffer P1HA 50mL, Buffer P2 40 mL, Buffer P3 40 mL, Buffer P4 6 mL, 50x Column P, 100x 2.0 mL collection tubes, 50x 1.5 mL collection tubes |
| Hazard Statements | Hazard Class 8 |
| Pack Size | 50 Preps |
| Personal Protective Equipment | Safety Goggles/Faceshield; Gloves |
| Sample Type | 25 - 200 µL DNA preparations > 200 bp |
| Storage | Store at Room Temperature (15-25℃) in a dry environment |
| Usage Statement | Research Use Only (RUO) |
![MP COA [116548050] lot ID: FC090F](https://www.mpbio.com/static/version1747301781/frontend/Mpbio/default/en_US/images/document.png "MP COA [116548050] lot ID: FC090F")