Product Description
Fibronectin
Application Notes
Human fibronectin (HFN) is suitable for use as an attachment factor in the propagation of cells in vitro when used to coat cell culture surfaces, including plasticware, glassware, and microcarrier beads. It is used in cell attachment and proliferation assays on human endothelial cells, human keratinocytes and human dermal fibroblasts.
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
Attachment factor
Specifications
| SKU | 0215112601 |
| Alternate Names | CIG; Cold insoluble globulin; Fibronectin from human plasma |
| Application Notes | Human fibronectin (HFN) is suitable for use as an attachment factor in the propagation of cells in vitro when used to coat cell culture surfaces, including plasticware, glassware, and microcarrier beads. It is used in cell attachment and proliferation assays on human endothelial cells, human keratinocytes and human dermal fibroblasts. |
| Base Catalog Number | 15112601 |
| CAS # | 86088-83-7 |
| EC Number | 289-149-2 |
| Format | Solid |
| Pack Size | 1 mg |
| Personal Protective Equipment | Eyeshields, Gloves, respirator filter |
| Preparation Method | HFN is purified from Cohn fraction I of human plasma by a modification of the procedure. This material has been pasteurized to reduce the possibility of viral contamination, and filtered through a 0.22 micron filter prior to lyophilization. |
| Purity | ≥95% |
| Source | Human plasma |
| Typical Working Concentration | Recommended for use as a cell culture substratum at 1-5 μg/cm2 or 0.5-50 μg/mL. Optimal concentration depends on cell type as well as the application or research objectives |
| Usage Statement | Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. |
Documents
Certificate of Analysis (COA)
![MP COA [151126] lot ID: QR11516](https://www.mpbio.com/static/version1747301781/frontend/Mpbio/default/en_US/images/document.png "MP COA [151126] lot ID: QR11516")
Datasheet
Datasheet
Brochures
Brochures