Fibronectin
Application Notes
Fibronectin has been shown to stimulate the proliferation of rat osteoblasts in vitro; to induce odontoblast polarization and to regulate the differentiation of human dental pulp cells into odontoblasts. It has wide applications in cellular research into proliferation, differentiation, development and morphology. Precoating of glass, plastic and micro-carrier beads aids cell attachment, particularly those cell which are deficient in cell surface fibronectin. It has been reported that proteolytic digests of fibronectin are mitogenic for CH23 fibroblasts.
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
Glycoproteins
| SKU | 02150025-CF |
| Alternate Names | CIG; Cold insoluble globulin |
| Application Notes | Fibronectin has been shown to stimulate the proliferation of rat osteoblasts in vitro; to induce odontoblast polarization and to regulate the differentiation of human dental pulp cells into odontoblasts. It has wide applications in cellular research into proliferation, differentiation, development and morphology. Precoating of glass, plastic and micro-carrier beads aids cell attachment, particularly those cell which are deficient in cell surface fibronectin. It has been reported that proteolytic digests of fibronectin are mitogenic for CH23 fibroblasts. |
| Base Catalog Number | 150025 |
| Biochemical Physiological Actions | Domains of fibronectin bind with collagen, fibrin, heparin, and specific cell membrane receptors. The most notable domain, Arg-Gly-Asp can be recognized by integrins and mediates cell adhesion. Fibronectin is also widely involved in interactions such as the attachment and migration of many cell types, cytoskeletal assembly, tyrosine phosphorylation, and metastasis. |
| CAS # | 86088-83-7 |
| EC Number | 289-149-2 |
| Extinction Coefficient | E1%= 12.9 (280 nm in 8 M urea-0.2 M NaOH)(Lit.) |
| Format | Lyophilized powder |
| Isoelectric Point | 5.5 - 6.3 (Lit.) |
| Personal Protective Equipment | Eyeshields, Gloves, Respirator filter |
| Purity | ≥90% |
| Source | Bovine Plasma |
| Sterility | Sterile Filtered |
| Typical Working Concentration | Cell culture substratum at 1-5 μg/cm2 or 0.5-50 μg/mL |
| Usage Statement | Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. |
![MP COA [150025] lot ID: M5270](https://www.mpbio.com/static/version1747301781/frontend/Mpbio/default/en_US/images/document.png "MP COA [150025] lot ID: M5270")
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