LymphoSep™, lymphocyte separation medium

Lymphosep - Lymphocyte Separation Medium is a sterile, iso-osmotic, low viscosity solution for density centrifugation yielding a clear separation of lymphocytes from heparinized or defibrinated human blood. Lymphocytes are isolated by layering diluted, heparinized blood over a solution of sodium diatrizoate that contains a polysaccharide. This polysaccharide aggregates the erythrocytes so that they can be sedimented by low centrifugation. The less dense lymphocytes remain as a clearly defined band at the plasma Lymphosep interface.

• Maximum yield of mononuclear cells
• Easy one-step centrifugation
• >96% cell viability of lymphocytes
• Low endotoxins
• Autoclave sterilization

See our latest article in Genetic Engineering & Biotechnology News about High-Performance Density Gradient Media for Purification of Leukocytes from Blood.

Application Notes

LSM is formulated for isolation of mononuclear cells from defibrinated or heparinized whole human blood. One-step centrifugation permits separation of mononuclear lymphocytes from erythrocytes, polynuclear lymphocytes and most platelets.

Usage Statement

Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.

Key Applications

In vitro isolation of lymphocytes from diluted whole blood.