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Reagent kits are reproducible, lot-to-lot consistency, scalability, and easy to use
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Evaluate 20 samples per hour, with as little as 10 - 50 μL sample volume
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Each kit is configured and standardized for analysis of 110 samples at a cost of $4.5 per sample
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Malonaldehyde Test Kits are designed to measure malonaldehyde content in oils, tallows, greases, feed, and protein meals in both raw materials and finished products. Malonaldehydes are an indicator of secondary oxidation associated with off odors and off flavors (rancidity).

  • Malonaldehyde Value Overview
  • Malonaldehyde Test Kits
  • Malonaldehyde Analysis
  • Malonaldehyde Test Kit Protocols
Malonaldehyde Analysis with Malonaldehyde Test Kit


What is Malonaldehyde?

Lipid peroxides and their hydrogen stripping powers will eventually degrade into other smaller products. One of the new products formed by these free radicals is malonaldehyde. Levels of malonaldehyde will increase in rancid foods. Malonaldehydes have both off odors and flavors, making them an excellent indicator of rancidity. While scientific evidence has yet to find a direct link between malonaldehyde and cancer, it is considered a potential carcinogen. The basic structure of malonaldehyde is presented below. Note the three-carbon backbone like a triglyceride. One can imagine how the breakdown of a triglyceride can result in a malonaldehyde.


What is Malonaldehyde Test Kit?

The Malonaldehyde Test Kits are designed to provide rapid analysis of the malonaldehyde content of oils, tallows, greases, feed, and protein meals using micro-analytical and membrane separation principles. The Malonaldehyde Test Kit quantitates malonaldehyde (MDA) through a condensation reaction where 2 MDA molecules react with a chromagen whose visible spectrum then changes and is read using the SafTest Analyzer. The proprietary Malonaldehyde Test can be used for testing solubilized dry and wet food matrices by releasing lipid from the sample matrix using a stabilized reagent (i.e. stabilized isopropanol) and employing mechanical mixing and warming techniques. The solubilized food matrix is then filtered through a membrane and the filtrate is analyzed for MDA. The short sample preparation time of 15 to 20 minutes permits data to be obtained in 25 to 30 minutes. MP Biomedical's Malonaldehyde Test Kit contains all reagents, calibrators, and controls required for testing up to 110-120 tests.


Sample Preparation: Liquid oils may require heating or dilution Semi-solid/solid oils and meals require heating and mechanical disruption. Meals require membrane separation
Time Requirement: Sample Preparation 5 to 20 minutes
Analysis time approximately 20 minutes
Limit of Quantitation: 0-60mg/kg or 0-1,200µmol/kg MDA
Interferences: Highly colored samples
Malonaldehyde Test Kits


The Malonaldehyde Test Kit is designed to provide rapid analysis of the malonaldehyde content of oils, tallows, greases, feed, and protein meals using micro-analytical and membrane separation principles. The Malonaldehyde Test Kit quantitates malonaldehyde (MDA) through a condensation reaction where 2 MDA molecules react with a chromagen whose visible spectrum then changes and is read using the SafTest Analyzer. The proprietary Malonaldehyde Test Kit can be used for testing solubilized dry and wet food matrices by releasing lipids from the sample matrix using a stabilized reagent (i.e. stabilized isopropanol) and employing mechanical mixing and warming techniques. The solubilized food matrix is then filtered through a membrane and the filtrate is analyzed for MDA. The short sample preparation time of 15 to 20 minutes permits data to be obtained in 25 to 30 minutes. MP Biomedical's Malonaldehyde Test Kit contains all reagents and calibrators required for testing up to 120 tests. The MDA value is expressed as µmol/kg or nmol/kg of sample.



Methodology

To demonstrate method repeatability, accuracy, and linearity, samples of various types of meals were analyzed using the AldeSafe Kit.


Repeatability and Accuracy Studies

Quality Profile on Meat and Bone Meal Samples

Quality Profile on Fish Meal

SafTest Results for Pet Food Samples Stored at 50°C for 52 to 194 days

AldeSafe Protocols






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