SPINeasy DNA Kit for Blood is a high-performance genomic DNA (gDNA) extraction kit based on silica-membrane spin-column technology. This kit enables quick isolation of gDNA from whole blood preserved in different anticoagulants (EDTA, Heparin and sodium citrate), typically in less than 30 minutes. It also allows easy gDNA isolation from other samples, including plasma, serum, saliva, and cell culture medium. The use of our specially formulated Lysis Buffer BL omits the need of homogenization and enables highly efficient lysis of various samples. Provided in the kit, Column S1 and kit buffers are designed to deliver gDNA extracts of high yield and purity, compatible with downstream applications such as PCR amplification.
Performance
SPINeasy DNA Kit for Blood has been tested on various sample types for its performance. The following data demonstrate the gDNA yields obtained from various sample sources. Results showed that all extracted gDNA are of high yield and purity (Table 1, Figure 1, and Figure 2) which suitable for downstream PCR amplification (Figure 3).
Table 1: Quality and quantity of gDNA extracted from various sample types using SPINeasy DNA kit for Blood.
*Sample processed using homogenization method via Fastprep-24TM 5G with lysing matrix.
**Sample preserved in preservation buffer (SPS, provided in SPINeasy DNA Kit for Saliva)
Application Notes
SPINeasy DNA Kit for Blood uses spin column technology for isolation of gDNA from variety of sample sources which include fresh, frozen, and anticoagulated blood, plasma, serum, and cell cultured medium.
Usage Statement
Research Use Only (RUO)
Key Applications
Genomics; Molecular Biology; PCR, qPCR, Microbiome analysis, NGS; shotgun sequencing
| SKU | 116552050 |
| Alternate Names | Spin-column DNA Extraction Kit from Blood, gDNA Extraction kit for blood |
| Application Notes | SPINeasy DNA Kit for Blood uses spin column technology for isolation of gDNA from variety of sample sources which include fresh, frozen, and anticoagulated blood, plasma, serum, and cell cultured medium. |
| Base Catalog Number | 52050 |
| Product Line | SPINeasy |
| Components | Lysis Buffer BL, Wash Buffer BW1, Wash Buffer BW2, Elution Buffer EB, Column S, 2.0 mL collection tubes,1.5 mL microcentrifuge tubes, Proteinase K, Proteinase K Buffer |
| Format | Kit |
| Pack Size | 50 Preps |
| Personal Protective Equipment | Gloves |
| Sample Type | Blood, Saliva, Cultured Cells |
| Sample Volume | 200 μL of Human blood, serum, plasma, saliva, or cell culture media |
| Storage | 15-25 ℃ |
| Usage Statement | Research Use Only (RUO) |
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