Product Description
Auramine O
Application Notes
Auramine O is used for acid resistant bacteria in sputum or paraffin.It is used with rhodamine B, causing acid-fast organisms to fluoresce. Used with acridine orange, showing acid-fast organisms and fungi, mycobacteria. (Auramine O is reportedly specific for mycolic acid). It is used as a Schiff reagent, causing DNA in nuclei to fluoresce yellow-green and is used with safranin O chloride to stain spores in bacterial cultures.
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
Stain for acid resistant bacteria in sputum or paraffin
Specifications
| SKU | 0219506450 |
| Alternate Names | C.I. 41000; 4,4-(Imidocarbonyl)Bis(N,N-Dimethyl-Amine) Monohydrochloride; Basic Yellow 2; Pyoctaninum aureum; Canary yellow |
| Application Notes | Auramine O is used for acid resistant bacteria in sputum or paraffin.It is used with rhodamine B, causing acid-fast organisms to fluoresce. Used with acridine orange, showing acid-fast organisms and fungi, mycobacteria. (Auramine O is reportedly specific for mycolic acid). It is used as a Schiff reagent, causing DNA in nuclei to fluoresce yellow-green and is used with safranin O chloride to stain spores in bacterial cultures. |
| Base Catalog Number | 19506450 |
| Beilstein Registry Number | 4030061 |
| CAS # | 2465-27-2 |
| Color Index | 41000 |
| EC Number | 219-567-2 |
| Format | Powder |
| Hazard Statements | H302-H311-H351 |
| Melting Point | 513° F (NTP, 1992) |
| Molecular Formula | C17H21N3·HCl·H2O |
| Molecular Weight | 303.834 g/mol |
| Pack Size | 50 g |
| RTECS Number | BY3675000 |
| Safety Symbol | GHS06, GHS08 |
| Solubility | less than 1 mg/mL at 64° F (NTP, 1992) |
| Usage Statement | Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. |
| UV Visible Absorbance | λmax (water) 432 ± 3 nm |
Documents
Certificate of Analysis (COA)
![MP COA [195064] lot ID: MR28804](https://www.mpbio.com/static/version1781680552/frontend/Mpbio/luma/en_US/images/document.png "MP COA [195064] lot ID: MR28804")
Datasheet
Datasheet
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