}{RIBONUCLEASE, BEEF PANCREAS

}{RIBONUCLEASE, BEEF PANCREAS

Synonyms RNase A; E.C. 2.7.7.16; Ribonuclease I; Ribonucleate 3′-pyrimidinooligonucleotidohydrolase; Pancreatic Ribonuclease; Endoribonuclease I

CAS Number: 9001-99-4 Molecular Weight: ~13,700 (Lit.)

EC Number: 232-646-6 MDL Number: MFCD00132181

Packaging size: 100 mg

SKU 02101075.1
Price:
$74.80
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Description

Product Description

RNase A is an endoribonuclease that attacks at the 3' phosphate of a pyrimidine nucleotide. The sequence of pG-pG-pC-pA-pG will be cleaved to give pG-pG-pCp and A-pG. The highest activity is exhibited with single stranded RNA. RNase A is a single chain polypeptide containing 4 disulfide bridges.

Application Notes

Ribonuclease A is used to remove RNA from DNA plasmid preparations and protein samples. It is also used in RNA sequence analysis and protection assays.

Ribonuclease A is used to remove RNA from DNA plasmid preparations and protein samples. Ribonuclease A is used for RNase protection assays, to remove unspecifically bound RNA, analysis of RNA sequences, to hydrolyze RNA contained in protein samples, and the purification of DNA. Ribonuclease A from bovine pancreas has been used in a study to assess the tissue-specific expression of ruminant pancreatic type ribonucleases. Ribonuclease A from bovine pancreas has also been used in a study to investigate pancreas-specific glycosylated protein disulphide-isomerase binding to misfolded proteins and peptides.

Usage Statement

Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Key Applications

Purification of DNA||RNA sequence analysis||RNase protection assays||Remove unspecifically bound RNA||Hydrolyze RNA contained in protein samples

Specifications
SKU 02101075.1
Alternate Names RNase A; E.C. 2.7.7.16; Ribonuclease I; Ribonucleate 3′-pyrimidinooligonucleotidohydrolase; Pancreatic Ribonuclease; Endoribonuclease I
Application Notes Ribonuclease A is used to remove RNA from DNA plasmid preparations and protein samples. It is also used in RNA sequence analysis and protection assays.<br><br>Ribonuclease A is used to remove RNA from DNA plasmid preparations and protein samples. Ribonuclease A is used for RNase protection assays, to remove unspecifically bound RNA, analysis of RNA sequences, to hydrolyze RNA contained in protein samples, and the purification of DNA. Ribonuclease A from bovine pancreas has been used in a study to assess the tissue-specific expression of ruminant pancreatic type ribonucleases. Ribonuclease A from bovine pancreas has also been used in a study to investigate pancreas-specific glycosylated protein disulphide-isomerase binding to misfolded proteins and peptides.
Biochemical Physiological Actions Ribonuclease A cleaves single stranded RNA after pyrimidine nucleotides.
EC Number 232-646-6
Extinction Coefficient E1%280 = 7.3(Lit.)
Hazard Statements H317
Applications Purification of DNA||RNA sequence analysis||RNase protection assays||Remove unspecifically bound RNA||Hydrolyze RNA contained in protein samples
MDL # MFCD00132181
Molecular Weight ~13,700 (Lit.)
Pack Size 100 mg
Personal Protective Equipment Eyeshields, Gloves, respirator filter
pH Optimum pH: 7.0 - 7.5(Lit.)
Physical Appearance Off-white Powder
Product Families Description RNase A is an endoribonuclease that attacks at the 3' phosphate of a pyrimidine nucleotide. The sequence of pG-pG-pC-pA-pG will be cleaved to give pG-pG-pCp and A-pG. The highest activity is exhibited with single stranded RNA. RNase A is a single chain polypeptide containing 4 disulfide bridges.
Related Products 02101076||02152024||02193980
RTECS Number RF0760000
Safety Phrases S:36
Solubility Dissolves readily at 2 mg/mL in analytical grade water to give a clear colorless solution.
Specific Activity ~70 Kunitz u/mg solid
Storage and Handling Store at -0 °C. Store Desiccated.
Unit Definition One unit causes the hydrolysis of RNA at a rate such that the velocity constant (k) equals 1 at 25 °C and pH 5.0.
Usage Statement Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.