Protease

Improve Sequence Coverage with Protease V8 for Protein Identification

Successful peptide mapping requires selection of the right cleavage enzymes. Protease V8 (also known as endoproteinase Glu-C) complements tryptic digestion, specifically cleaving peptide bonds on the C-terminal side of glutamic and aspartic acids. Protease V8 from MP Bio has been validated by more than 200 scientific publications and provides:

• Robust activity with greater than 915 units/mg protein
• Specific cleavage at glutamic acid residue in ammonium bicarbonate at pH 7.8, ammonium acetate at pH 4 and specific digestion at both glutamic and aspartic acid residues in phosphate buffer at pH 7.8
• Complement to tryptic digestion
• High tolerance over a broad range of buffers, pHs and denaturing reagents
• Lyophilized format for longer stability and easier storage