Proteins & Enzymes
Purified Mouse Myeloma Protein IgM (Lambda1) MOPC 104E
These myeloma proteins are purified from ascites by a combination of salt precipitation, ion exchange and bioaffinity chromatography. Purified proteins are dialyzed into 0.02M Tris, 0.14M sodium chloride, pH 8.1. Product is adjusted to 1.0 mg/ml, filtered, vialed, and stored frozen at -70°C. Product is shipped on dry ice.
Goat IgG, Lyophilized
This product is useful for structural and biological studies, as a reference standard, or as starting material for the preparation of an immunogen.
It is supplied in Tris or phosphate buffered saline.
See Purified Immunoglobulins and Myeloma Proteins in the Immunobiologicals section for further details.
Horse IgG (Lyophilized)
Horse IgG is useful for structural and biological studies, as a reference standard, or as starting material for immunogen preparation. It is supplied purified in Tris or phosphate buffered saline. Each vial contains 100 mg of lyophilized protein based on 1% E 280nm = 14 The product was lyophilized from 0.01 M phosphate buffer with 0.15 M sodium chloride, pH 7.4. Procedure:The immunoglobulin was prepared from horse serum using current chromatographic
Tryptase (Purified Antigen)
Tryptase is a glycoprotein released from mast cells during anaphylaxis, which performs a number of functions including catalyzing the activation of complement C3, converting prostromelysin to stromelysin (MMP-3), and cleaving fibrinogen resulting in a loss of clotting potential. Human tryptase is a major secretory protease of human lung mast cells.
(1,4-β-Xylan xylohydrolase, EC 184.108.40.206)
From Trichoderma viride
Activity: 5 U/vial
Unit Definition: One unit is defined as the amount of enzyme required to liberate 1 μmole of p-nitrophenol from p-nitrophenyl-α-D-xylopyranoside per minute at 37°C, pH 4.0.
Human Albumin Fraction V
This human protein is prepared by a modification of the Cohn procedure. Albumin may be used to eliminate background interference in ELISA's or other enzyme assay systems. It is also used in absorption, distribution, metabolism, and excretion (ADME) pharmacological research; cell culture; drug delivery research; and cryopreservation of cells. Human and bovine albumins contain 16% nitrogen and are often used as standards in protein calibration studies. Due to their free hydrophobic region fatty acid free albumins are used to solubilize lipids in tissue culture, and are also used as blocking agents in Western blots or ELISA applications. Globulin free albumins are suitable for use in applications where no other proteins should be present (e.g., electrophoresis). Serum albumin functions as a carrier protein for steroids, fatty acids, and thyroid hormones, and is vital in regulating the colloidal osmotic pressures of blood. Albumin is also seen to bind to exogenous substances, particularly drugs (e.g., ibuprofen, warfarin), and strongly influence their pharmacokinetics. Oxidative stress leading to changes in the redox state of albumin has widely varied effects on its physiological function.