Cell Separation Media, Mono/Poly Resolving Media
High Performance Density Gradient Media for Mononuclear and Polymorphonuclear Leukocyte Separation
Isolation of viable mononuclear and polymorphonuclear cells from blood serves as the starting point for a wide spectrum of immunology and cell biology studies. Early methods for isolating leukocytes were often unsatisfactory as the cells would become trapped by, and sedimented with, the aggregated erythrocytes, resulting in broad spectrum damage to the isolated cells. In 1968, a more convenient and rapid separation was introduced using centrifugation through a Ficoll-sodium metrizoate solution. This separation method takes advantage of cell density differences of the components in whole blood that, when centrifuged in the presence of a density gradient media, exhibits a unique migration pattern through the medium, allowing distinct cell populations to be fractionated. The whole process is performed at a lower gravitational force field without the introduction of aggregating reagents, thus preserving cell viability.
MP Bio offers three products for the isolation of mononuclear and polymorphonuclear cells from human peripheral blood as well as bone marrow, and umbilical cord blood. Lymphocyte Separation Medium (LSM™), LymphoSep®, and Mono-Poly® Resolving Medium have been used for many applications by researchers worldwide.
Mono-Poly Resolving Medium
Mono-Poly® Resolving Medium separates mononuclear and polymorphonuclear leukocytes from whole human blood in two distinct bands. Mono-Poly™ is highly versatile and can be used for a variety of research and clinical applications such as histocompatibility testing, lymphocyte studies, in vitro cell mediated immunity assays, or any procedure requiring pure white blood cell preparation. This product provides: • Separation of mononuclear and polymorphonuclear leukocytes in two distinct bands. • Greater than 95% cell viability. • An easy, single step procedure. • No sample dilution requirement or lysis of red blood cells