Secondary Antibodies

Secondary Antibodies

Specific Secondary Antibodies from Cappel® for Sensitive Detection and Purification

Secondary antibodies are designed to bind to primary antibodies to amplify signals for detection, separation and quantification of target antigen. To maximize signal, a secondary antibody must have specificity to interact with the primary antibody species and isotype. In addition, secondary antibodies are typically conjugated with a reporter molecule, which may include an enzyme like horseradish peroxidase (HRP) or a fluorophore such as fluorescein isothiocyanate (FITC). Multiple secondary antibodies can bind to a single primary antibody, leading to amplified signal and sensitivity.

Cappel secondary antibodies from MP Bio are available with or without enzyme/fluorescence dye conjugation from multiple immunoglobulins, including human, rabbit and mouse. Enzyme conjugated antibodies (alkaline phosphatase (AP) or HRP) are suitable for EIA, ELISA, blot immunostaining and cell/tissue staining, and fluorochrome conjugated antibodies are used in immunofluorescence assays, cell/tissue staining, blot immunostaining and fluorescence-activated cell sorting.

MP Bio secondary antibodies from Cappel have been validated by thousands of publications since the 1960's and offer:

  • High specificity for primary antibody species and isotypes
  • Multiple pre-conjugations for sensitive detection
  • Various fragments
  • High purity
189 Results Found

Goat IgG Fraction To Human IgG Fc

Product is the lyophilized powder of goat IgG fraction to human IgG Fc and buffer salts.

Monoclonal Mouse Anti-Human IgG (Gamma Chain) (Clone: GG-5)

Anti-HumanClone: GG-5Isotype: mouse IgG1 kappaConc/Titer: 1:20Applications: PAP; immunohistochemistry; paraffin-embedded and frozen sectionsImmunohistology GradeThis antibody reacts to

Rabbit Antiserum To Human IgG (Whole Molecule)

Anti-Human
Host:
rabbit
Form: lyophilized
Applications: Immunostaining of acetone-fixed frozen and formalin-fixed, paraffin-embedded tissue sections.

Goat IgG Fraction To Human IgG Fab

Product is the lyophilized powder of goat IgG fraction to human IgG Fab and buffer salts. The IgG fraction is prepared from the specific goat antiserum by delipidation, ammonium sulfate fractionation and anion exchange chromatography. The resulting IgG fraction is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, adjusted to standard titer, filtered through a a 0.22 µm filter, vialed and lyophilized.

Rabbit IgG Fraction To Human IgG (Whole Molecule)

Product is the lyophilized powder of rabbit IgG fraction to human IgG (whole molecule) and buffer salts. The IgG fraction is prepared from the specific rabbit antiserum by delipidation, ammonium sulfate fractionation and anion exchange chromatography. The resulting IgG fraction is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, adjusted to standard titer, filtered through a 0.22 µm filter, vialed and lyophilized.

Sheep IgG Fraction To Human IgG3

The product is Sheep IgG fraction (1ml liquid) to human IgG3 containing 0.1% sodium azide in Tris/saline, pH 7.4.

Sheep Anti-Human IgG4 (Lyophilized)

The human IgG4 antiserum was produced by immunizing sheep with highly purified IgG4 myeloma. Antiserum has been rendered specific for the IgG4 subclass determinants using suitable immunoadsorbents. The antiserum is an IgG fraction enriched whole sheep serum.

Mouse Anti-Human IgM Monoclonal (Clone: HP6083)

Monoclonal anti-human IgM is conjugated with amino-hexanoyl-biotin N-hydroxysuccininimide under optimum conditions and is free of unconjugated biotin.

Goat Antiserum To Human IgM (5Fc µ)

Anti-Human
Host:
goat
Form: lyophilized
Applications: Immunostaining of acetone-fixed frozen and formalin-fixed, paraffin-embedded tissue sections.

Goat F(Ab')2 Fragment To Human IgM (5Fc µ)

Product is the lyophilized powder of goat F(ab')2 fragment to human IgM (5Fc μ) and buffer salts.

Goat IgG Fraction To Human IgM (5Fc µ)

Product is the lyophilized powder of goat IgG fraction to human IgM (5Fc μ) and buffer salts.

Goat Affinity Purified Antibody To Human IgM (5Fc µ)

Product is goat affinity purified antibody to human IgM (5Fc μ) and buffer salts.

Goat F(Ab')2 Fragment To Human Kappa Chain (Bound)

Product is the lyophilized powder of goat F(ab')2 fragment to human kappa chain (bound) and buffer salts.

Goat Affinity Purified Antibody To Mouse IgA (Alpha Chain)

Product is goat affinity purified antibody to mouse IgA (a chain) and buffer salts

Goat Affinity Purified Antibody To Mouse IgG (Whole Molecule)

Product is goat affinity purified antibody to mouse IgG (whole molecule) and buffer salts.

Goat Affinity Purified Antibody To Mouse IgG Fc

Product is goat affinity purified antibody to mouse IgG Fc and buffer salts.

Goat Antiserum To Mouse IgG (Whole Molecule)

Product is the lyophilized powder of goat antiserum to mouse IgG (whole molecule) and buffer salts.

Goat Affinity Purified F(Ab')2 Fragment To Mouse IgG (Whole Molecule)

Product is goat affinity purified F(ab')2 fragments to mouse IgG (whole molecule) and buffer salts.

Goat IgG Fraction To Mouse IgG (Whole Molecule)

Product is the lyophilized powder of goat IgG fraction to mouse IgG (whole molecule) and buffer salts.

Goat IgG Fraction To Mouse IgG Fc

Product is the lyophilized powder of goat IgG fraction to mouse IgG Fc and buffer salts.