Secondary Antibodies

Secondary Antibodies

Specific Secondary Antibodies from Cappel® for Sensitive Detection and Purification

Secondary antibodies are designed to bind to primary antibodies to amplify signals for detection, separation and quantification of target antigen. To maximize signal, a secondary antibody must have specificity to interact with the primary antibody species and isotype. In addition, secondary antibodies are typically conjugated with a reporter molecule, which may include an enzyme like horseradish peroxidase (HRP) or a fluorophore such as fluorescein isothiocyanate (FITC). Multiple secondary antibodies can bind to a single primary antibody, leading to amplified signal and sensitivity.

Cappel secondary antibodies from MP Bio are available with or without enzyme/fluorescence dye conjugation from multiple immunoglobulins, including human, rabbit and mouse. Enzyme conjugated antibodies (alkaline phosphatase (AP) or HRP) are suitable for EIA, ELISA, blot immunostaining and cell/tissue staining, and fluorochrome conjugated antibodies are used in immunofluorescence assays, cell/tissue staining, blot immunostaining and fluorescence-activated cell sorting.

MP Bio secondary antibodies from Cappel have been validated by thousands of publications since the 1960's and offer:

  • High specificity for primary antibody species and isotypes
  • Multiple pre-conjugations for sensitive detection
  • Various fragments
  • High purity
189 Results Found

Rhodamine-Conjugated Goat IgG Fraction To Mouse IgG (Whole Molecule)

Product is the lyophilized powder of tetramethylrhodamine-5-(and-6)-isothiocyanate (TRITC)-conjugated goat IgG fraction to mouse IgG (whole molecule) and buffer salts.

Rhodamine-Conjugated Goat Affinity Purified Antibody To Rabbit IgG (Whole Molecule)

Product is tetramethylrhodamine-5-(and-6)-isothiocyanate (TRITC)-conjugated goat affinity purified antibody to rabbit IgG (whole molecule) and buffer salts.

Rhodamine-Conjugated Rabbit IgG Fraction To Rat IgG (Whole Molecule)

Product is the lyophilized powder of tetramethylrhodamine-5- (and-6)- isothiocyanate (TRITC)-conjugated rabbit IgG fraction to rat IgG (whole molecule) and buffer salts.

Alkaline Phosphatase-Conjugated Goat Affinity Purified Antibody To Human IgG Fc (No Cross Horse)

Product is alkaline phosphatase (ALP)-conjugated goat affinity purified antibody to human IgG Fc and buffer salts

Goat Anti-Human IgM (Fc5U Fragment) Affinity Purified, ALK Conjugated

Anti-Human
Host:
goat
Form: affinity purified lyophilized
Applications: ELISA and immunoblotting.
Alkaline Phosphatase Conjugated

Alkaline Phosphatase-Conjugated Goat Affinity Purified Antibody To Human Immunoglobulins (IgG, IgA, IgM)

Product is alkaline phosphatase (AKP)-conjugated goat affinity purified antibody to human immunoglobulins (IgG,IgA,IgM) and buffer salts.

Alkaline Phosphatase-Conjugated Goat Affinity Purified Antibody To Rabbit IgG (Whole Molecule)

Product is alkaline phosphatase (AKP)-conjugated goat affinity purified antibody to rabbit IgG (whole molecule) and buffer salts.

Alkaline Phosphatase-Conjugated Goat Affinity Purified Antibody To Rabbit IgG Fc

Product is alkaline phosphatase (AKP)-conjugated goat affinity purified antibody to rabbit IgG Fc and buffer salts.

Rabbit Anti-Sheep IgG (Fc Fragment) Affinity Purified, Alkaline Phosphatase Conjugated

Anti-Goat
Host:
rabbit
Form: affinity purified, lyophilized
Applications: immunoblotting and ELISA.
Alkaline Phosphatase Conjugated

Goat Affinity Purified Antibody To Cat IgG (Whole Molecule)

Product is goat affinity purified antibody to cat IgG (whole molecule) and buffer salts.

Goat Affinity Purified F(Ab')2 Fragment To Human IgG Fc

Product is goat affinity purified F(ab')2 fragments to human IgG Fc and buffer salts. The affinity purified F(ab')2 fragment is prepared from the specific goat IgG fraction by limited digestion with pepsin and solid phase absorption. The resulting affinity purified F(ab')2 fragment is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, adjusted to standard titer, filtered through a 0.22 µm filter, and vialed.

Goat Affinity Purified F(Ab')2 Fragment To Human IgM (5Fc Mu)

: Product is goat affinity purified F(ab')2 fragments to human IgM (5Fc µ) and buffer salts. The affinity purified F(ab')2 fragment is prepared from the specific goat IgG fraction by limited digestion with pepsin and solid phase absorption. The resulting affinity purified F(ab')2 fragment is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, adjusted to standard titer, filtered through a 0.22 µm filter, and vialed.

Rabbit Antiserum To Bovine IgG (Whole Molecule)

Product is the lyophilized powder of rabbit antiserum to bovine IgG (whole molecule) and buffer salts. The antiserum is prepared by hyperimmunizing rabbits with highly purified bovine IgG; antiserum is delipidated and made specific by solid-phase absorption.

Rabbit IgG Fraction To Bovine IgG (Whole Molecule)

Product is the lyophilized powder of rabbit IgG fraction to bovine IgG (whole molecule) and buffer salts

Fluorescein-Conjugated Goat Affinity Purified Antibody To Cat IgG (Whole Molecule)

Product is fluorescein-5-isothiocyanate (FITC "Isomer I")-conjugated goat affinity purified antibody to cat IgG (whole molecule) and buffer salts.

Fluorescein-Conjugated Goat IgG Fraction To Cat IgG (Whole Molecule)

Product is the lyophilized powder of fluorescein-5-isothiocyanate (FITC "Isomer I")-conjugated goat IgG fraction to cat IgG (whole molecule) and buffer salts

Peroxidase-Conjugated Goat IgG Fraction To Cat IgG (Whole Molecule)

Product is the lyophilized powder of horseradish peroxidase (HRP)-conjugated goat IgG fraction to cat IgG (whole molecule) and buffer salts.

Rabbit IgG Fraction To Chicken Red Blood Cells

Product is the lyophilized powder of rabbit IgG fraction to chicken red blood cells and buffer salts.

Goat Antiserum To Dog IgG (Whole Molecule)

Product is the lyophilized powder of goat antiserum to dog IgG (whole molecule) and buffer salts

Rabbit Antiserum To Dog IgG (Whole Molecule)

Product is the lyophilized powder of Rabbit Antiserum to Dog IgG (whole molecule) and buffer salts.