Secondary Antibodies

Secondary Antibodies

Specific Secondary Antibodies from Cappel® for Sensitive Detection and Purification

Secondary antibodies are designed to bind to primary antibodies to amplify signals for detection, separation and quantification of target antigen. To maximize signal, a secondary antibody must have specificity to interact with the primary antibody species and isotype. In addition, secondary antibodies are typically conjugated with a reporter molecule, which may include an enzyme like horseradish peroxidase (HRP) or a fluorophore such as fluorescein isothiocyanate (FITC). Multiple secondary antibodies can bind to a single primary antibody, leading to amplified signal and sensitivity.

Cappel secondary antibodies from MP Bio are available with or without enzyme/fluorescence dye conjugation from multiple immunoglobulins, including human, rabbit and mouse. Enzyme conjugated antibodies (alkaline phosphatase (AP) or HRP) are suitable for EIA, ELISA, blot immunostaining and cell/tissue staining, and fluorochrome conjugated antibodies are used in immunofluorescence assays, cell/tissue staining, blot immunostaining and fluorescence-activated cell sorting.

MP Bio secondary antibodies from Cappel have been validated by thousands of publications since the 1960's and offer:

  • High specificity for primary antibody species and isotypes
  • Multiple pre-conjugations for sensitive detection
  • Various fragments
  • High purity
189 Results Found

Peroxidase-Conjugated Goat IgG Fraction To Mouse IgG (Whole Molecule)

Product is the lyophilized powder of horseradish peroxidase (HRP)-conjugated goat IgG fraction to mouse IgG (whole molecule) and buffer salts. Anti-Mouse IgG is developed in goat using purified mouse IgG as the immunogen. Affinity isolation removes essentially all goat serum proteins, including immunoglobulins which do not specifically bind to mouse IgG.

Peroxidase-Conjugated Goat IgG Fraction To Mouse IgG F(Ab')2

Product is the lyophilized powder of horseradish peroxidase (HRP)-conjugated goat IgG fraction to mouse IgG F(ab')2 and buffer salts.

Peroxidase-Conjugated Goat IgG Fraction To Mouse IgG Fc

Product is the lyophilized powder of horseradish peroxidase (HRP)-conjugated goat IgG fraction to mouse IgG Fc and buffer salts.

Peroxidase-Conjugated Rabbit Affinity Purified Antibody To Mouse IgG (Whole Molecule)

Product is horseradish peroxidase (HRP)-conjugated rabbit affinity purified antibody to mouse IgG (whole molecule) and buffer salts.

Biotin-Conjugated Sheep Affinity Purified F(Ab')2 Fragment To Mouse IgG (No Cross To Human)

Product is biotin-conjugated sheep affinity purified F(ab')2 fragment to mouse IgG (whole molecule) in a stabilized solution.

Peroxidase-Conjugated Sheep IgG Fraction To Mouse IgG (No Cross To Human)

Product is the lyophilized powder of horseradish peroxidase (HRP)-conjugated sheep IgG fraction to mouse IgG (no cross to human) and buffer salts.

Peroxidase-Conjugated Goat IgG Fraction To Mouse IgM (µ Chain)

Anti-Mouse
Host:
goat
Form: lyophilized IgG
Applications: Immunostaining of acetone-fixed frozen and formalin-fixed, paraffin-embedded tissue sections.
HRP Conjugated

Alkaline Phosphatase-Conjugated Rabbit Affinity Purified Antibody To Goat IgG (Whole Molecule)

Product is alkaline phosphatase (AKP)-conjugated rabbit affinity purified antibody to goat IgG (whole molecule) and buffer salts.

Goat Anti-Rabbit IgG (H+L)

Liquid. This product is goat anti-sera with 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, containing 0.05% sodium azide as a preservative.