Secondary Antibodies, HRP conjungates

Secondary Antibodies

Specific Secondary Antibodies from Cappel® for Sensitive Detection and Purification

Secondary antibodies are designed to bind to primary antibodies to amplify signals for detection, separation and quantification of target antigen. To maximize signal, a secondary antibody must have specificity to interact with the primary antibody species and isotype. In addition, secondary antibodies are typically conjugated with a reporter molecule, which may include an enzyme like horseradish peroxidase (HRP) or a fluorophore such as fluorescein isothiocyanate (FITC). Multiple secondary antibodies can bind to a single primary antibody, leading to amplified signal and sensitivity.

Cappel secondary antibodies from MP Bio are available with or without enzyme/fluorescence dye conjugation from multiple immunoglobulins, including human, rabbit and mouse. Enzyme conjugated antibodies (alkaline phosphatase (AP) or HRP) are suitable for EIA, ELISA, blot immunostaining and cell/tissue staining, and fluorochrome conjugated antibodies are used in immunofluorescence assays, cell/tissue staining, blot immunostaining and fluorescence-activated cell sorting.

MP Bio secondary antibodies from Cappel have been validated by thousands of publications since the 1960's and offer:

  • High specificity for primary antibody species and isotypes
  • Multiple pre-conjugations for sensitive detection
  • Various fragments
  • High purity
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Rabbit IgG Fraction To Horseradish Peroxidase

Product is the lyophilized powder of rabbit IgG fraction to horseradish peroxidase and buffer salts.

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