Secondary Antibodies, Unconjugated

Secondary Antibodies

Specific Secondary Antibodies from Cappel® for Sensitive Detection and Purification

Secondary antibodies are designed to bind to primary antibodies to amplify signals for detection, separation and quantification of target antigen. To maximize signal, a secondary antibody must have specificity to interact with the primary antibody species and isotype. In addition, secondary antibodies are typically conjugated with a reporter molecule, which may include an enzyme like horseradish peroxidase (HRP) or a fluorophore such as fluorescein isothiocyanate (FITC). Multiple secondary antibodies can bind to a single primary antibody, leading to amplified signal and sensitivity.

Cappel secondary antibodies from MP Bio are available with or without enzyme/fluorescence dye conjugation from multiple immunoglobulins, including human, rabbit and mouse. Enzyme conjugated antibodies (alkaline phosphatase (AP) or HRP) are suitable for EIA, ELISA, blot immunostaining and cell/tissue staining, and fluorochrome conjugated antibodies are used in immunofluorescence assays, cell/tissue staining, blot immunostaining and fluorescence-activated cell sorting.

MP Bio secondary antibodies from Cappel have been validated by thousands of publications since the 1960's and offer:

  • High specificity for primary antibody species and isotypes
  • Multiple pre-conjugations for sensitive detection
  • Various fragments
  • High purity
72 Results Found

Goat Affinity Purified Antibody To Cat IgG (Whole Molecule)

Product is goat affinity purified antibody to cat IgG (whole molecule) and buffer salts.

Goat Affinity Purified F(Ab')2 Fragment To Human IgG Fc

Product is goat affinity purified F(ab')2 fragments to human IgG Fc and buffer salts. The affinity purified F(ab')2 fragment is prepared from the specific goat IgG fraction by limited digestion with pepsin and solid phase absorption. The resulting affinity purified F(ab')2 fragment is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, adjusted to standard titer, filtered through a 0.22 µm filter, and vialed.

Goat Affinity Purified F(Ab')2 Fragment To Human IgM (5Fc Mu)

: Product is goat affinity purified F(ab')2 fragments to human IgM (5Fc µ) and buffer salts. The affinity purified F(ab')2 fragment is prepared from the specific goat IgG fraction by limited digestion with pepsin and solid phase absorption. The resulting affinity purified F(ab')2 fragment is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, adjusted to standard titer, filtered through a 0.22 µm filter, and vialed.

Rabbit Antiserum To Bovine IgG (Whole Molecule)

Product is the lyophilized powder of rabbit antiserum to bovine IgG (whole molecule) and buffer salts. The antiserum is prepared by hyperimmunizing rabbits with highly purified bovine IgG; antiserum is delipidated and made specific by solid-phase absorption.

Rabbit IgG Fraction To Bovine IgG (Whole Molecule)

Product is the lyophilized powder of rabbit IgG fraction to bovine IgG (whole molecule) and buffer salts

Rabbit IgG Fraction To Chicken Red Blood Cells

Product is the lyophilized powder of rabbit IgG fraction to chicken red blood cells and buffer salts.

Goat Antiserum To Dog IgG (Whole Molecule)

Product is the lyophilized powder of goat antiserum to dog IgG (whole molecule) and buffer salts

Rabbit Antiserum To Dog IgG (Whole Molecule)

Product is the lyophilized powder of Rabbit Antiserum to Dog IgG (whole molecule) and buffer salts.

Rabbit Anti-Dog IgG (H+L) Affinity Purified

This antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Goat Antiserum To Dog IgM (µ Chain)

Product is the lyophilized powder of goat antiserum to dog IgM (µ chain) and buffer salts

Rabbit Anti-Dog IgM Serum

Prepared in rabbits with highly purified normal dog IgM in Freunds adjuvant. Then the antiserum is solid phase absorbed to ensure specificity to the mu chain.

Rabbit, Anti-Goat IgG Fc Fragment, Affinity Purified

Anti-Goat
Host: rabbit
Form: affinity purified liquid
Applications: ELISA, IEP and gel diffusion

Donkey Anti-Goat IgG (H + L), Affinity Purified

Sterile liquid. The antibody is in 0.01M sodium phosphate, 0.25M NaCl, pH 7.6. No preservatives added.

Goat Affinity Purified Antibody To Hamster IgG (Whole Molecule)

Product is goat affinity purified antibody to hamster IgG (whole molecule) and buffer salts.

Rabbit Antiserum To Hamster IgG (Whole Molecule)

Product is the lyophilized powder of rabbit antiserum to hamster IgG (whole molecule) and buffer salts.

Rabbit IgG Fraction To Hamster IgG (Whole Molecule)

Anti-HamsterHost: rabbitForm: lyophilized (IgG)Applications: Immunostaining of acetone-fixed frozen and formalin-fixed, paraffin-embedded tissue sections.

Goat Antiserum To Horse IgG (Whole Molecule)

Product is the lyophilized powder of goat antiserum to horse IgG (whole molecule) and buffer salts

Rabbit IgG Fraction To Horseradish Peroxidase

Product is the lyophilized powder of rabbit IgG fraction to horseradish peroxidase and buffer salts.

Goat Antiserum To Human Secretory IgA

Product is the lyophilized powder of goat antiserum to human secretory IgA and buffer salts.

Goat Affinity Purified Antibody To Human IgA (Alpha Chain)

Product is goat affinity purified antibody to human IgA (α-chain) and buffer salts.