Secondary Antibodies, Biotin

Secondary Antibodies

Specific Secondary Antibodies from Cappel® for Sensitive Detection and Purification

Secondary antibodies are designed to bind to primary antibodies to amplify signals for detection, separation and quantification of target antigen. To maximize signal, a secondary antibody must have specificity to interact with the primary antibody species and isotype. In addition, secondary antibodies are typically conjugated with a reporter molecule, which may include an enzyme like horseradish peroxidase (HRP) or a fluorophore such as fluorescein isothiocyanate (FITC). Multiple secondary antibodies can bind to a single primary antibody, leading to amplified signal and sensitivity.

Cappel secondary antibodies from MP Bio are available with or without enzyme/fluorescence dye conjugation from multiple immunoglobulins, including human, rabbit and mouse. Enzyme conjugated antibodies (alkaline phosphatase (AP) or HRP) are suitable for EIA, ELISA, blot immunostaining and cell/tissue staining, and fluorochrome conjugated antibodies are used in immunofluorescence assays, cell/tissue staining, blot immunostaining and fluorescence-activated cell sorting.

MP Bio secondary antibodies from Cappel have been validated by thousands of publications since the 1960's and offer:

  • High specificity for primary antibody species and isotypes
  • Multiple pre-conjugations for sensitive detection
  • Various fragments
  • High purity
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Mouse Anti-Human IgM Monoclonal (Clone: HP6083)

Monoclonal anti-human IgM is conjugated with amino-hexanoyl-biotin N-hydroxysuccininimide under optimum conditions and is free of unconjugated biotin.

Mouse Anti-Human IgG (Clone HP6045) Biotinylated Monoclonal

Lyophilized powder. From 10 mM PBS, pH 7.4, containing 1% BSA and 0.05% sodium azide

Mouse Anti-Human IgG1 Biotinylated (Clone: HP6069) Monoclonal

Applications: immunoblotting and immunohistochemistry.

Biotin-Conjugated Goat Affinity Purified Antibody To Rat IgG (Whole Molecule)

Product is biotin-conjugated goat affinity purified antibody to rat IgG (whole molecule) in a stabilized solution.

Biotin-Conjugated Sheep Affinity Purified F(Ab')2 Fragment To Mouse IgG (No Cross To Human)

Product is biotin-conjugated sheep affinity purified F(ab')2 fragment to mouse IgG (whole molecule) in a stabilized solution.

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