Secondary Antibodies, Monoclonal
Specific Secondary Antibodies from Cappel® for Sensitive Detection and Purification
Secondary antibodies are designed to bind to primary antibodies to amplify signals for detection, separation and quantification of target antigen. To maximize signal, a secondary antibody must have specificity to interact with the primary antibody species and isotype. In addition, secondary antibodies are typically conjugated with a reporter molecule, which may include an enzyme like horseradish peroxidase (HRP) or a fluorophore such as fluorescein isothiocyanate (FITC). Multiple secondary antibodies can bind to a single primary antibody, leading to amplified signal and sensitivity.
Cappel secondary antibodies from MP Bio are available with or without enzyme/fluorescence dye conjugation from multiple immunoglobulins, including human, rabbit and mouse. Enzyme conjugated antibodies (alkaline phosphatase (AP) or HRP) are suitable for EIA, ELISA, blot immunostaining and cell/tissue staining, and fluorochrome conjugated antibodies are used in immunofluorescence assays, cell/tissue staining, blot immunostaining and fluorescence-activated cell sorting.
MP Bio secondary antibodies from Cappel have been validated by thousands of publications since the 1960's and offer:
- High specificity for primary antibody species and isotypes
- Multiple pre-conjugations for sensitive detection
- Various fragments
- High purity
Monoclonal Anti-Human IgG Fc Fragment (Clone HP6017)
Liquid, Monoclonal Anti-Human IgG Fc Fragment (Clone HP 6017) in 10 mM phosphate buffered saline, pH 7.4
Monoclonal Mouse Anti-Human IgG (Gamma Chain) (Clone: GG-5)
Anti-HumanClone: GG-5Isotype: mouse IgG1 kappaConc/Titer: 1:20Applications: PAP; immunohistochemistry; paraffin-embedded and frozen sectionsImmunohistology GradeThis antibody reacts to
Mouse Anti-Human IgG (Clone HP6045) Biotinylated Monoclonal
Lyophilized powder. From 10 mM PBS, pH 7.4, containing 1% BSA and 0.05% sodium azide
Mouse Anti-Human IgG1 Biotinylated (Clone: HP6069) Monoclonal
Applications: immunoblotting and immunohistochemistry.
Mouse Anti-Human IgG (Fd Region, All Sub-Classes) HRP Conjugated Monoclonal
The product is conjugated with highly purified horseradish peroxidase (RZ>3.0).
Mouse Anti-Human IgG1 HRP Conjugated (Clone HP6069) Monoclonal
Anti-HumanClone: HP6069Applications: ELISA and immunoblotting.HRP ConjugatedSpecies Reactivity:Human IgG SubclassesPeroxidaseHost: MousePurified, Liquid