Primary Antibodies, Mouse
Monoclonal Anti-Human B-Lymphocyte LN-3
LN-3 is a murine monoclonal antibody (IgG2b) reactive with a nonpolymorphic HLA-DR (Ia-like) antigen. The antigens show the typical 29-33 K dalton molecular weight of Ia. The HLA-DR antigen is present on germinal center and mantle zone B cells, monocytes and macrophages and interdigitating histiocytes. T cells are generally negative although activated T cells may express HLA-DR.
Anti-Prostatic Specific Antigen (PSA) Monoclonal ( Clone ER-PR8)
Isotype: mouse IgG1 presented as ascites fluid
Applications: Immunoblotting; Immunohistochemistry.
The original immunogen was from human prostate. The antibody reacts with a 34 kDa protein. Immunohistochemical studies have shown that the presence of PSA is highly specific for the demonstration of the prostatic origin of a malignancy. Antibodies to PSA have been shown to be valuable reagents for the positive identification of primary and metastatic prostatic carcinoma. This antibody reacts with both frozen and formalin-fixed paraffin embedded tissues.
Mouse Anti-Neurofilament 200 Monoclonal (Clone NE14)
The immunogen used to produce this monoclonal antibody is neurofilament purified from human brain. The isotype is mouse IgG1. This product is a monoclonal antibody to the neurofilament peptide of apparent molecular weight of 200,000.
Mouse Anti-Synaptophysin IgG1 Monoclonal (Clone: SY38)
The immunogen used to make this antibody is synaptophysin from presynaptic vesicles prepared from bovine brain. The mouse monoclonal antibody is then purified by Protein A affinity chromatography.
Vimentin Monoclonal Antibody From Mouse
Isotype: mouse IgG1
Applications: Immunoblotting; Immunostaining of cell suspensions, acetone-fixed frozen tissue sections, and formalin-fixed, paraffin-embedded (microwaved) tissue sections.
The original antigen was purified calf lens. The antibody reacts specifically to 57 kDa vimentin protein in immunoblotting. Additionally, it reacts specifically with cells of mesenchymal origin including fibroblasts, endothelial cells, smooth muscle cells, chondrocytes, lymphocytes and blood cells. It cross-reacts with vimentin of most mammals including human, mouse, rat and rabbit. It is supplied as clarified cell culture supernatant with 1% BSA and 0.09% sodium azide.
Ref.: Van Muijen, G.N., et al., Lab. Invest., 57(4): 359-369, (1987).
Anti-Human Collagen Type II, Mouse (Clone II-4CII), Purified IgG
Anti-human Type II collagen antibody was raised in mice after immunization with collagen Type II from human costal cartilage.
Mouse Anti-Actin, Monoclonal (Clone C4)
The monoclonal actin (clone C4) is a mouse IgG1 (gamma 1 heavy/kappa light chain) antibody. The monoclonal actin (clone C4) is a mouse IgG1 (gamma 1 heavy/kappa light chain) antibody. Chicken gizzard actin was used as the immunogen. Immunoblots demonstrate specificity directed towards all actins ranging from yeast, to flies, to mouse, to man. These are skeletal alpha-actin, cardiac alpha-actin, smooth muscle gamma actin, cytoplasmic beta-actin, cytoplasmic gamma actin as well as Dictyostelium discoideum and Physarum olycephalum actins. The epitope for the antibody lies between residues 18-40, a highly conserved region of the molecule. This antibody can also be used for cells cultivated on slides, for frozen tissue sections and as a loading control for western blots.
Mouse Anti-Glial Fibrillary Acidic Protein (GFAP) Monoclonal
Product is a mouse monoclonal antibody, isotype IgG1, that reacts with Glial Fibrillary Acidic Protein.
Monoclonal Mouse Anti-Chondroitin-4-Sulfate Antibody
Reacts with both mouse (fetal and adult) and human (fetal and adult) chondroitinase ABC digested cartilage proteoglycans. Chondroitin-4 sulfate disaccharides successfully inhibit the binding of monoclonal antibody to proteoglycans.
Monoclonal Mouse Anti-Chondroitin-6-Sulfate Antibody
Reacts with a wide range of cartilage proteoglycans after either chondroitinase (ABC or AC) or testicular hyaluronidase digestion.
Monoclonal Anti-Alpha-Smooth Muscle Actin (Mouse Ascites Fluid), Clone 1A4
Anti-α-Smooth Muscle Actin
Isotype: mouse IgG2a ascites
Applications: Indirect Immunofluorescence.
The original immunogen was the NH2 terminal synthetic decapeptide of α smooth muscle actin coupled to KLH. It is specific for the single isoform α smooth muscle actin. In the original publication describing this antibody it was named anti-alpha-sm-1.
Mouse Anti-Actin Mab Clone B4
The monoclonal actin (clone B4) antibody is a mouse IgG1, kappa light chain antibody. Chicken gizzard was used as the immunogen. Immunoblots demonstrate specificity directed towards the smooth muscle gamma-enteric isoform of actin. This antibody can be used to detect the other smooth muscle actin (alpha-skeletal, alpha-cardiac and alpha-vascular) found in vertebrates.