Mammalian Media

Mammalian Media

High Quality and Consistent Animal Sera for Optimal Cell Culture

Animal sera, whether from bovine, human or other animal source, provides all essential nutrients such as proteins, attachment factors, growth factors, amino acids, trace elements, vitamins, lipids and hormones for healthy cell culture growth. It plays an essential role in regulating cell osmotic pressure and membrane permeability, serving as a carrier for enzymes, micronutrients, lipids and trace elements into the cell. Animal sera has been widely used as a nutrient boost for most cell culture applications in the life sciences.

To meet and exceed quality control standards for high performance in cell culture, all sera are tested by independent third-party laboratories for the presence of endotoxins, mycoplasma, bacteria, fungi and viruses. Additional testing is performed for total protein concentration (including hemoglobin content) and cell growth. A certificate of analysis for each lot is available upon request. Our animal sera ensure:

  • High performance for broad cell types
  • Low endotoxin levels
  • Free of mycoplasma contamination
  • Free of disease from animal sources
  • Minimized lot-to-lot variability
  • Sterility
  • Country of origin and traceability

Fetal Bovine Serum

Fetal bovine serum (FBS) is one of the most highly implemented serum supplements for in vitro cell culture. This is due to its complex array of protein components, the presence of optimal cell growth factors, low endotoxin levels and low hemoglobin concentration, all of which are required by cells to sustain, grow and divide. FBS offers excellent value for basic cell culture applications, specialty research and bioprocessing. Our quality assurance standards ensure reliable and consistent delivery of high quality FBS from raw material to final product. Heat inactivated CELLect™ FBS Gold is the industry standard for FBS supplements.

Sera from Other Animals

Although fetal bovine serum is the most commonly used sera for cell culture, several other animal sera alternatives can be selected based on cell origin, cross-reactivity, performance requirements and costs, including human serum, newborn calf serum, horse serum, goat serum, rabbit serum, porcine serum and chicken serum. A wide spectrum of animal sera are available to meet your cell culture needs for both research and bioprocessing applications.

67 Results Found

Medium 199 With Earle's Salt (E199), Powder, With L-Glutamine, Without Sodium Bicarbonate

Medium 199, originally described by Morgan and his colleagues (1950), is a completely defined nutritional source for cell culture. Their investigations demonstrated that cell growth could be measured in this medium. It has broad species applicability including the culturing of non-transformed cell types. It may be used for vaccine production and the in vitro cultivation of rat lens tissues and primary mouse pancreatic epithelial explants. It is available in either Earle's or Hanks' salts.

Medium 199 With Hanks' Salts (H199), Powder, With L-Glutamine Without Sodium Bicarbonate

Medium 199, originally described by Morgan and his colleagues (1950), is a completely defined nutritional source for cell culture. Their investigations demonstrated that cell growth could be measured in this medium. It has broad species applicability including the culturing of non-transformed cell types. It may be used for vaccine production and the in vitro cultivation of rat lens tissues and primary mouse pancreatic epithelial explants. It is available in either Earle's or Hanks' salts.

Dulbecco's Modification Of Eagle's Medium (DMEM), Powder, With 4500 mg/L Dextrose, L-Glutamine, Without Sodium Bicarbonate

Dulbecco's Modified Eagle's Medium (DMEM) is the most widely used modification of Eagle's Basal Medium (BME). DMEM contains four times greater concentration of amino acids, vitamins and supplementary components. The original formulation calls for 1000 mg/L of glucose, which was first employed to support the polyoma virus in primary and secondary embryonic mouse cultures. For optimal culturing of other cell types, a modification of 4500 mg/L glucose is available.

Biorich W/Gln

BioRich 1 is a DMEM/F-12 powdered media blend enriched with trace elements. This permits the transition to "serum-free" conditions reducing or eliminating the necessity for serum supplementation. It is used with ITS Premix, a completely serum independent.

RPMI 1640, With L-Glutamine, Without Sodium Bicarbonate

This medium was originally developed by Moore and his colleagues at Roswell Park Memorial Institute (RPMI). It was based on the RPMI 1630 line of media which utilized a bicarbonate buffering system and alterations in the amounts of amino acids and vitamins. RPMI has successfully been used for the cultivation of normal human and neoplastic leukocytes. It is now a popular general purpose medium when properly supplemented.

Minimum Essential Medium Eagle (Modified) With Earle's Salts Without L-Glutamine Or Sodium Bicarbonate

MEM, originally prepared by Harry Eagle, is one of the most popular cell culture media. Upon his attempts to cultivate normal mammalian fibroblasts and certain HeLa cell subtypes, it was revealed that the nutritional needs of these cell types could not be met by BME. Further studies led to the development of MEM incorporating specific modifications such as higher amino acid concentrations for the cultivation of fastidious cells. MP's MEM may be used to support the growth of cells in monolayers, in suspension and wide variety of other cell types with proper supplementation. MP offers MEM with either Earle's or Hanks' salts.