Mammalian Media, Mammalian Media

Mammalian Media

High Quality and Consistent Animal Sera for Optimal Cell Culture

Animal sera, whether from bovine, human or other animal source, provides all essential nutrients such as proteins, attachment factors, growth factors, amino acids, trace elements, vitamins, lipids and hormones for healthy cell culture growth. It plays an essential role in regulating cell osmotic pressure and membrane permeability, serving as a carrier for enzymes, micronutrients, lipids and trace elements into the cell. Animal sera has been widely used as a nutrient boost for most cell culture applications in the life sciences.

To meet and exceed quality control standards for high performance in cell culture, all sera are tested by independent third-party laboratories for the presence of endotoxins, mycoplasma, bacteria, fungi and viruses. Additional testing is performed for total protein concentration (including hemoglobin content) and cell growth. A certificate of analysis for each lot is available upon request. Our animal sera ensure:

  • High performance for broad cell types
  • Low endotoxin levels
  • Free of mycoplasma contamination
  • Free of disease from animal sources
  • Minimized lot-to-lot variability
  • Sterility
  • Country of origin and traceability

Fetal Bovine Serum

Fetal bovine serum (FBS) is one of the most highly implemented serum supplements for in vitro cell culture. This is due to its complex array of protein components, the presence of optimal cell growth factors, low endotoxin levels and low hemoglobin concentration, all of which are required by cells to sustain, grow and divide. FBS offers excellent value for basic cell culture applications, specialty research and bioprocessing. Our quality assurance standards ensure reliable and consistent delivery of high quality FBS from raw material to final product. Heat inactivated CELLect™ FBS Gold is the industry standard for FBS supplements.

Sera from Other Animals

Although fetal bovine serum is the most commonly used sera for cell culture, several other animal sera alternatives can be selected based on cell origin, cross-reactivity, performance requirements and costs, including human serum, newborn calf serum, horse serum, goat serum, rabbit serum, porcine serum and chicken serum. A wide spectrum of animal sera are available to meet your cell culture needs for both research and bioprocessing applications.

67 Results Found

Active filters:

1X RPMI Without L-Glutamine, L-Cysteine, L-Cystine, and L-Methionine

RPMI 1640 Medium was originally developed by Moore and his colleagues at Roswell Park Memorial Institute (RPMI). It was based on the RPMI 1630 line of media which utilized a bicarbonate buffering system and alterations in the amounts of amino acids and vitamins. A Dutch Modification of RPMI 1640 is also available through MP. The Dutch Modification contains 6400 mg/L sodium chloride instead of 6000 mg/L; contains 1000 mg/L sodium bicarbonate instead of 2000 mg/L; and contains 20 mM HEPES.

1X RPMI 1640 Without L-Glutamine and Phosphate, With 0.85 g/L Sodium Bicarbonate

RPMI 1640 Medium was originally developed by Moore and his colleagues at Roswell Park Memorial Institute (RPMI). It was based on the RPMI 1630 line of media which utilized a bicarbonate buffering system and alterations in the amounts of amino acids and vitamins. A Dutch Modification of RPMI 1640 is also available through MP. The Dutch Modification contains 6400 mg/L sodium chloride instead of 6000 mg/L, 1000 mg/L sodium bicarbonate instead of 2000 mg/L, and 20 mM HEPES.

1X RPMI 1640 Without L-Glutamine, Phenol Red

This medium was originally developed by Moore and his colleagues at Roswell Park Memorial Institute (RPMI). It was based on the RPMI 1630 line of media which utilized a bicarbonate buffering system and alterations in the amounts of amino acids and vitamins. RPMI has successfully been used for the cultivation of normal human and neoplastic leukocytes. It is now a popular general purpose medium when properly supplemented. Without L-glutamine, phenol red; Endotoxin Tested; Sterile Filtered

RPMI 1640 (1X Solution) Without L-Glutamine and L-Leucine

RPMI 1640 was originally developed by Moore and his colleagues at Roswell Park Memorial Institute (RPMI). It was based on the RPMI 1630 line of media which utilized a bicarbonate buffering system and alterations in the amounts of amino acids and vitamins. A Dutch Modification of RPMI 1640 is also available through MP. The Dutch Modification contains 6400 mg/L sodium chloride instead of 6000 mg/L, 1000 mg/L sodium bicarbonate instead of 2000 mg/L, and 20 mM HEPES.

RPMI 1640 With 2 g/L Sodium Bicarbonate, Without L-Glutamine & Glucose

RPMI 1640 Medium was originally developed by Moore and his colleagues at Roswell Park Memorial Institute (RPMI). It was based on the RPMI 1630 line of media which utilized a bicarbonate buffering system and alterations in the amounts of amino acids and vitamins. A Dutch Modification of RPMI 1640 is also available through MP. The Dutch Modification contains 6400 mg/L sodium chloride instead of 6000 mg/L; contains 1000 mg/L sodium bicarbonate instead of 2000 mg/L; and contains 20 mM HEPES.

RPMI 1640 Without L-Glutamine

RPMI 1640 (1X Solution) without L-glutamine, pH 6.9 to 7.2. This medium was originally developed by Moore and his colleagues at Roswell Park Memorial Institute (RPMI). It was based on the RPMI 1630 line of media which utilized a bicarbonate buffering system and alterations in the amounts of amino acids and vitamins. RPMI has successfully been used for the cultivation of normal human and neoplastic leukocytes. It is now a popular general purpose medium when properly supplemented.

0916820.png

100 mM Sodium Pyruvate (11.00 mg/mL)

Sodium pyruvate is used by cells as an easily accessible carbohydrate source. Additionally, it is involved with amino acid metabolism and initiates the Kreb′s cycle. The 100 mM solution should be diluted 1:100 for most cell culture. The use of sodium pyruvate in Wallen fermentation medium to enhance the conversion of oleic acid to 10-ketostearic acid by Bacillus sphaericus has been described. A protocol that uses sodium pyruvate to establish stably transfected human B cell lines has been published. It improves coliform recovery when present in culture medium.

Chicken Embryo Extract 20 mL

Chicken embryo extract is prepared from 9-12 day old eggs collected from registered flocks and found to be free from clinical signs of notifiable diseases.

Hat Supplement (50X Solution)

(50X Concentrate)Hypoxanthine 13.61 mg/LAminopterin 2H2O 0.1906 mg/LThymidine 3.876 mg/LSelective for hybridoma cell growth and growth of cells with a functioning HGPRT mechanism. The main application is for the selective growth of hybridoma cells for monoclonal antibody production.

HT (50X Solution)

(50X Concentrate) Hypoxanthine 13.61 mg/L Thymidine 3.876 mg/L This media is selective for growth of cells with a functioning HGPRT mechanism. The main application is for the selective growth of hybridoma cells for monoclonal antibody production.

ITS Premix Solution

ITS Premix will stimulate cell proliferation while decreasing substantially the serum requirements for culture of many cell types as diverse, for example, as contractile rat heart cells and human colon mucosal epithelial cells. Basal media supplemented with ITS Premix and as little as 2% Fetal Bovine Serum support proliferation of many diploid and heteroploid cell lines at rates equivalent to those obtained with 10% serum. Contains 0.5 mg/ml insulin from bovine pancreas, 0.5 mg/ml human transferrin (substantially iron-free) and 0.5 ug/ml sodium selenite. Prepared in Earle's balanced Salt Solution (EBSS) without phenol red. Sterile-filtered.

Williams Medium E, Powder, With L-Glutamine, Without Sodium Bicarbonate

Isolated epithelial cells, as described in his sequential plating method by Williams, et al., in 1971, were originally cultivated in a rich medium known as Williams' Medium D. From these early newborn animal studies, Williams and Gunn developed a subsequent medium, Williams' Medium E, for the long-term cultivation of adult rat liver epithelial cells.

Alpha Modification Of Eagle's Medium (AMEM), Powder, With L-Glutamine, Without Deoxyribosides, Ribosides, Sodium Bicarbonate

Alpha Modification of Eagle's Medium (AMEM), powder, with L-glutamine, without deoxyribosides, ribosides, sodium bicarbonate. Originally used for growth of hamster kidney cells.

Ham's F-12 (Modified) With L-Glutamine, Without Sodium Bicarbonate

Originally, Ham's Nutrient Mixtures were developed for the clonal growth of several Chinese hamster ovary (CHO) clone cells, Hela cells and mouse L-cells. Both F-10 and F-12 are formulated for use with or without serum, depending on the type of cells being cultured. F-10 has demonstrated satisfactory growth of human diploid cells, white blood cells for chromosomal analysis and primary rat, rabbit and chicken tissue explants. Ham's F-12 is well suited for the growth of primary rat hepatocytes and prostate epithelial cells. It is also the medium of choice for toxicity assays using CHO cells. MP also offers Ham's F-12 supplemented with 25 mM HEPES for improved buffering in the optimum pH range of 7.2-7.4. F12K is a modification of Ham's F-12 and Coon's F-12 with increased amino acid and pyruvate concentrations. Additionally, the salts have been modifed (Konigsbergs). F12K has been developed for culturing differentiated rat and chicken cells, as well as, primary human liver cells. MgCl2 has been substituted by MgSO4 containing an equivalent amount of Mg ion.

Hanks' Balanced Salts, Powder, Without Sodium Bicarbonate

Hanks' Balanced Salts solution is used to maintain the pH and osmotic balance in the medium and to provide the cells with water and essential inorganic ions.

Phosphate Buffered Saline (PBS), Dulbecco's Formula, Powder, Without Calcium, Magnesium

Phosphate Buffered Salts, Dulbecco's Formula (DPBS) is a balanced salt solution (BSS) used for the handling and culturing of mammalian cells. DPBS is used to irrigate, wash, and dilute mammalian cells. Phosphate buffering maintains the pH in the physiological range. Calcium and magnesium facilitate cell binding and clumping. DPBS without these ions can be used to wash and rinse suspended cells.

Newborn Bovine Serum (NBS) 

This serum is produced from blood collected from newly born calves between 1-14 days of age.

Minimum Essential Medium Eagle (Modified) (1X Solution) Without L-Glutamine

MEM, originally prepared by Harry Eagle, is one of the most popular cell culture media. Upon his attempts to cultivate normal mammalian fibroblasts and certain HeLa cell subtypes, it was revealed that the nutritional needs of these cell types could not be met by BME. Further studies led to the development of MEM incorporating specific modifications such as higher amino acid concentrations for the cultivation of fastidious cells. MP's MEM may be used to support the growth of cells in monolayers, in suspension and wide variety of other cell types with proper supplementation. MP offers MEM with either Earle's or Hanks' salts.

Dulbecco's Modified Eagle's Medium (DMEM) (1X Solution) With 20 mM HEPES, Without L-Glutamine Or Sodium Bicarbonate

Also known as DME, Dulbecco's Modified Eagle's Medium is the most widely used modification of Eagle's Basal Medium (BME). DMEM contains four times greater concentration of amino acids, vitamins and supplementary components. The original formulation calls for 1000 mg/L of glucose, which was first employed to support the polyoma virus in primary and secondary embryonic mouse cultures. For optimal culturing of other cell types, a modification of 4500 mg/L glucose is available from MP.

RPMI 1640 (1X Solution) With L-Glutamine With Sodium Bicarbonate

RPMI 1640 Medium was originally developed by Moore and his colleagues at Roswell Park Memorial Institute (RPMI). It was based on the RPMI 1630 line of media which utilized a bicarbonate buffering system and alterations in the amounts of amino acids and vitamins. A Dutch Modification of RPMI 1640 is also available through MP. The Dutch Modification contains 6400 mg/L sodium chloride instead of 6000 mg/L, 1000 mg/L sodium bicarbonate instead of 2000 mg/L, and 20 mM HEPES.