Mammalian Media, Culture Media

Mammalian Media

High Quality and Consistent Animal Sera for Optimal Cell Culture

Animal sera, whether from bovine, human or other animal source, provides all essential nutrients such as proteins, attachment factors, growth factors, amino acids, trace elements, vitamins, lipids and hormones for healthy cell culture growth. It plays an essential role in regulating cell osmotic pressure and membrane permeability, serving as a carrier for enzymes, micronutrients, lipids and trace elements into the cell. Animal sera has been widely used as a nutrient boost for most cell culture applications in the life sciences.

To meet and exceed quality control standards for high performance in cell culture, all sera are tested by independent third-party laboratories for the presence of endotoxins, mycoplasma, bacteria, fungi and viruses. Additional testing is performed for total protein concentration (including hemoglobin content) and cell growth. A certificate of analysis for each lot is available upon request. Our animal sera ensure:

  • High performance for broad cell types
  • Low endotoxin levels
  • Free of mycoplasma contamination
  • Free of disease from animal sources
  • Minimized lot-to-lot variability
  • Sterility
  • Country of origin and traceability

Fetal Bovine Serum

Fetal bovine serum (FBS) is one of the most highly implemented serum supplements for in vitro cell culture. This is due to its complex array of protein components, the presence of optimal cell growth factors, low endotoxin levels and low hemoglobin concentration, all of which are required by cells to sustain, grow and divide. FBS offers excellent value for basic cell culture applications, specialty research and bioprocessing. Our quality assurance standards ensure reliable and consistent delivery of high quality FBS from raw material to final product. Heat inactivated CELLect™ FBS Gold is the industry standard for FBS supplements.

Sera from Other Animals

Although fetal bovine serum is the most commonly used sera for cell culture, several other animal sera alternatives can be selected based on cell origin, cross-reactivity, performance requirements and costs, including human serum, newborn calf serum, horse serum, goat serum, rabbit serum, porcine serum and chicken serum. A wide spectrum of animal sera are available to meet your cell culture needs for both research and bioprocessing applications.

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Alsever's Solution

Alsever’s solution is an isotonic, balanced salt solution.

Basal Medium Eagle (BME) Vitamin Concentrate (100X)

100X BME, Modified
Vitamins
Without L-glutamine
Shipped frozen with dry ice.

10X Trypsin HBSS Without Calcium and Magnesium

Trypsin is a porcine pancreas-derived enzyme that is commonly used for the dissociation and disaggregation of anchorage-dependent mammalian cells and tissues. The concentration of trypsin necessary to dislodge cells from their substrate is dependent primarily on the cell type and the age of the culture.

Dulbecco's Modification Of Eagle's Medium (1X Solution) With 4.5 g/L Dextrose, Without L-Glutamine and Inositol

Also known as DME, Dulbecco's Modified Eagle's Medium is the most widely used modification of Eagle's Basal Medium (BME). DMEM contains four times greater concentration of amino acids, vitamins and supplementary components. The original formulation calls for 1000 mg/L of glucose, which was first employed to support the polyoma virus in primary and secondary embryonic mouse cultures. For optimal culturing of other cell types, a modification of 4500 mg/L glucose is available from MP

Dulbecco's Modification Of Eagle's Medium (1X Solution) Without L-Glutamine, Leucine, Sodium Pyruvate

Also known as DME, Dulbecco's Modified Eagle's Medium is the most widely used modification of Eagle's Basal Medium (BME). DMEM contains four times greater concentration of amino acids, vitamins and supplementary components. The original formulation calls for 1000 mg/L of glucose, which was first employed to support the polyoma virus in primary and secondary embryonic mouse cultures. For optimal culturing of other cell types, a modification of 4500 mg/L glucose is available from MP.

Dulbecco's Modification Of Eagle's Medium (DMEM) (1X Solution) Without L-Glutamine, Phenol Red

Also known as DME, Dulbecco's Modified Eagle's Medium is the most widely used modification of Eagle's Basal Medium (BME). DMEM contains four times greater concentration of amino acids, vitamins and supplementary components. The original formulation calls for 1000 mg/L of glucose, which was first employed to support the polyoma virus in primary and secondary embryonic mouse cultures. For optimal culturing of other cell types, a modification of 4500 mg/L glucose is available from MP.

Dulbecco's Modification Of Eagle's Medium (DMEM), Powder, With 4500 mg/L Dextrose, L-Glutamine, Without Sodium Bicarbonate, Sodium Pyruvate

Dulbecco's Modification of Eagle's Medium, with L-Glutamine, without Sodium Bicarbonate, without Sodium Pyruvate

Dulbecco's Modification Of Eagle's Medium With L-Glutamine and 4.5 g/L Glucose

Also known as DME, Dulbecco's Modified Eagle's Medium is the most widely used modification of Eagle's Basal Medium (BME). DMEM contains four times greater concentration of amino acids, vitamins and supplementary components. The original formulation calls for 1000 mg/L of glucose, which was first employed to support the polyoma virus in primary and secondary embryonic mouse cultures. For optimal culturing of other cell types, a modification of 4500 mg/L glucose is available from MP. S

Earle's Balanced Salts (10X Solution) Without Sodium Bicarbonate

Earle's Balanced Salts (10X Solution) without sodium bicarbonate

Earle's Balanced Salts (Modified) (EBS) (1X Solution) Without Phenol Red

Earle's Balanced Salts (Modified) (EBS) (1X Solution) without phenol red

Earle's Balanced Salts Without Sodium Bicarbonate

Earle's Balanced Salts without Sodium Bicarbonate. Particle Size: >90% thru 100 mesh pH (with 2200 mg/L NaHCO3): 7.57 Osmolality (with 2200 mg/L NaHCO3): 289 mM/kg Endotoxin: <0.10 EU/ml Mycoplasma: Negative

Minimum Essential Medium Eagle (Modified) (1X Solution) With Hank's Salts, 0.35 g/L Sodium Bicarbonate Without L-Glutamine

MEM, originally prepared by Harry Eagle, is one of the most popular cell culture media. Upon his attempts to cultivate normal mammalian fibroblasts and certain HeLa cell subtypes, it was revealed that the nutritional needs of these cell types could not be met by BME. Further studies led to the development of MEM incorporating specific modifications such as higher amino acid concentrations for the cultivation of fastidious cells. MP's MEM may be used to support the growth of cells in monolayers, in suspension and wide variety of other cell types with proper supplementation. MP offers MEM with either Earle's or Hanks' salts.

Minimum Essential Medium Eagle (Modified) (1X Solution) With Hank's Salts, 20 mM HEPES, Without L-Glutamine, Sodium Bicarbonate

MEM, originally prepared by Harry Eagle, is one of the most popular cell culture media. Upon his attempts to cultivate normal mammalian fibroblasts and certain HeLa cell subtypes, it was revealed that the nutritional needs of these cell types could not be met by BME. Further studies led to the development of MEM incorporating specific modifications such as higher amino acid concentrations for the cultivation of fastidious cells. MP's MEM may be used to support the growth of cells in monolayers, in suspension and wide variety of other cell types with proper supplementation. MP offers MEM with either Earle's or Hanks' salts.

Minimum Essential Medium Eagle With Earle's Salts and 2.0 g/L Sodium Bicarbonate Without L-Glutamine, L-Cysteine, L-Cystine, and L-Methionine

MEM, originally prepared by Harry Eagle, is one of the most popular cell culture media. Upon his attempts to cultivate normal mammalian fibroblasts and certain HeLa cell subtypes, it was revealed that the nutritional needs of these cell types could not be met by BME. Further studies led to the development of MEM incorporating specific modifications such as higher amino acid concentrations for the cultivation of fastidious cells. MP's MEM may be used to support the growth of cells in monolayers, in suspension and wide variety of other cell types with proper supplementation. MP offers MEM with either Earle's or Hanks' salts.

High Growth Enhancement Medium, With L-Glutamine, Without Sodium Bicarbonate

High Growth Enhancement Medium is a modification of DMEM in which 3.6 g/liter fructose is substituted for glucose, resulting in better pH control and higher cell yields. Fructose is metabolized slower than glucose, resulting in slower acid buildup.

Ham's F-10 (10X Solution) Without L-Glutamine, Without Sodium Bicarbonate, With 12.4 mg/L Phenol Red

Originally, Ham's Nutrient Mixtures were developed for the clonal growth of several Chinese hamster ovary (CHO) clone cells, Hela cells and mouse L-cells. Both F-10 and F-12 are formulated for use with or without serum, depending on the type of cells being cultured. F-10 has demonstrated satisfactory growth of human diploid cells, white blood cells for chromosomal analysis and primary rat, rabbit and chicken tissue explants. Ham's F-12 is well suited for the growth of primary rat hepatocytes and prostate epithelial cells. It is also the medium of choice for toxicity assays using CHO cells. MP also offers Ham's F-12 supplemented with 25 mM HEPES for improved buffering in the optimum pH range of 7.2-7.4. F12K is a modification of Ham's F-12 and Coon's F-12 with increased amino acid and pyruvate concentrations. Additionally, the salts have been modifed (Konigsbergs). F12K has been developed for culturing differentiated rat and chicken cells, as well as, primary human liver cells.

Ham's F-10, With L-Glutamine, Without Sodium Bicarbonate

Originally, Ham's Nutrient Mixtures were developed for the clonal growth of several Chinese hamster ovary (CHO) clone cells, Hela cells and mouse L-cells. Both F-10 and F-12 are formulated for use with or without serum, depending on the type of cells being cultured. F-10 has demonstrated satisfactory growth of human diploid cells, white blood cells for chromosomal analysis and primary rat, rabbit and chicken tissue explants. Ham's F-12 is well suited for the growth of primary rat hepatocytes and prostate epithelial cells. It is also the medium of choice for toxicity assays using CHO cells. MP also offers Ham's F-12 supplemented with 25 mM HEPES for improved buffering in the optimum pH range of 7.2-7.4. F12K is a modification of Ham's F-12 and Coon's F-12 with increased amino acid and pyruvate concentrations. Additionally, the salts have been modifed (Konigsbergs). F12K has been developed for culturing differentiated rat and chicken cells, as well as, primary human liver cells.