Mammalian Media, Frozen Liquid

Mammalian Media

High Quality and Consistent Animal Sera for Optimal Cell Culture

Animal sera, whether from bovine, human or other animal source, provides all essential nutrients such as proteins, attachment factors, growth factors, amino acids, trace elements, vitamins, lipids and hormones for healthy cell culture growth. It plays an essential role in regulating cell osmotic pressure and membrane permeability, serving as a carrier for enzymes, micronutrients, lipids and trace elements into the cell. Animal sera has been widely used as a nutrient boost for most cell culture applications in the life sciences.

To meet and exceed quality control standards for high performance in cell culture, all sera are tested by independent third-party laboratories for the presence of endotoxins, mycoplasma, bacteria, fungi and viruses. Additional testing is performed for total protein concentration (including hemoglobin content) and cell growth. A certificate of analysis for each lot is available upon request. Our animal sera ensure:

  • High performance for broad cell types
  • Low endotoxin levels
  • Free of mycoplasma contamination
  • Free of disease from animal sources
  • Minimized lot-to-lot variability
  • Sterility
  • Country of origin and traceability

Fetal Bovine Serum

Fetal bovine serum (FBS) is one of the most highly implemented serum supplements for in vitro cell culture. This is due to its complex array of protein components, the presence of optimal cell growth factors, low endotoxin levels and low hemoglobin concentration, all of which are required by cells to sustain, grow and divide. FBS offers excellent value for basic cell culture applications, specialty research and bioprocessing. Our quality assurance standards ensure reliable and consistent delivery of high quality FBS from raw material to final product. Heat inactivated CELLect™ FBS Gold is the industry standard for FBS supplements.

Sera from Other Animals

Although fetal bovine serum is the most commonly used sera for cell culture, several other animal sera alternatives can be selected based on cell origin, cross-reactivity, performance requirements and costs, including human serum, newborn calf serum, horse serum, goat serum, rabbit serum, porcine serum and chicken serum. A wide spectrum of animal sera are available to meet your cell culture needs for both research and bioprocessing applications.

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Mccoy's 5A Medium (Park & Terasaki Modification) (1X Solution) Without L-Glutamine, Sodium Bicarbonate

McCoy's 5A medium, as modified by Iwakata and Grace, is identical to RPMI 1629. Originally in 1959, McCoy and his colleagues described the amino acid requirements for the in vitro culturing of Novikoff Hepatoma cells. Basal Medium 5A was modified to create what is known as McCoy's 5A Medium for these investigations. Hsu and Kellogg further demonstrated the use of this medium to support the growth of primary cultures derived from normal bone marrow, testes, mouse kidney, skin, gingiva, rat embryo and other tissues. MP's medium is additionally suited for the propagation of leukocytes, biopsy tissues and the most demanding primary and continuous cell types. It is also available as modified by Park and Terasaki.

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