Mammalian Media

67 Results Found

RPMI 1640 (1X Solution) With L-Glutamine With Sodium Bicarbonate

RPMI 1640 Medium was originally developed by Moore and his colleagues at Roswell Park Memorial Institute (RPMI). It was based on the RPMI 1630 line of media which utilized a bicarbonate buffering system and alterations in the amounts of amino acids and vitamins. A Dutch Modification of RPMI 1640 is also available through MP. The Dutch Modification contains 6400 mg/L sodium chloride instead of 6000 mg/L, 1000 mg/L sodium bicarbonate instead of 2000 mg/L, and 20 mM HEPES.

Phosphate Buffered Saline (PBS) (1X Solution) Dulbecco's Formula Without Calcium, Magnesium

Phosphate Buffered Salts, Dulbecco's Formula (DPBS) is a balanced salt solution (BSS) used for the handling and culturing of mammalian cells. DPBS is used to irrigate, wash, and dilute mammalian cells. Phosphate buffering maintains the pH in the physiological range. Calcium and magnesium facilitate cell binding and clumping. DPBS without these ions can be used to wash and rinse suspended cells.

CELLect Fetal Bovine Serum, Silver, Mexico Origin

CELLect™ Silver is collected from a U.S.D.A. regulated and monitored abbatoir by technicians who work "on-site" where the fetal blood is aseptically collected via cardiac puncture. This raw material is transported to the production facility where it undergoes sterilization via filtration with multiple 0.1 micron filters. The care and diligence taken in handling the fetal serum minimizes cell lysis and other detrimental factors thereby reducing possible lysozyme and hemoglobin contamination. This translates into increased performance in the final product. It is supplied sterile, with no detectable mycoplasma or viral infection.

CELLect Fetal Bovine Serum, Gold, Us Origin

CELLect™ Gold is collected from a U.S.D.A. regulated and monitored abattoir by technicians who work "on-site" where the fetal blood is aseptically collected via cardiac puncture. This raw material is transported to the production facility where it undergoes sterilization via filtration through multiple 0.1 micron filters. The care and diligence taken in handling the fetal serum minimizes cell lysis and other detrimental factors thereby reducing possible lysozyme and hemoglobin contamination. This translates into increased performance in the final product. It is superior for hybridoma cloning. It is supplied sterile, with no detectable mycoplasma or viral infection. Serum is an extremely complex mixture of many small and large biomolecules with different, physiologically balanced growth-promoting and growth inhibiting activities.

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CELLect Fetal Bovine Serum, Gold, Heat Inactivated

Fetal bovine serum has been the most widely used growth supplement for cell culture media because of its high content of embryonic growth-promoting factors. When used at appropriate concentrations, it supplies many defined and undefined components that have been shown to satisfy specific metabolic requirements for the culture of cells in vitro.

Alsever's Solution

Alsever’s solution is an isotonic, balanced salt solution.

Basal Medium Eagle (BME) Vitamin Concentrate (100X)

100X BME, Modified
Vitamins
Without L-glutamine
Shipped frozen with dry ice.

10X Trypsin HBSS Without Calcium and Magnesium

Trypsin is a porcine pancreas-derived enzyme that is commonly used for the dissociation and disaggregation of anchorage-dependent mammalian cells and tissues. The concentration of trypsin necessary to dislodge cells from their substrate is dependent primarily on the cell type and the age of the culture.

Dulbecco's Modification Of Eagle's Medium (1X Solution) With 4.5 g/L Dextrose, Without L-Glutamine and Inositol

Also known as DME, Dulbecco's Modified Eagle's Medium is the most widely used modification of Eagle's Basal Medium (BME). DMEM contains four times greater concentration of amino acids, vitamins and supplementary components. The original formulation calls for 1000 mg/L of glucose, which was first employed to support the polyoma virus in primary and secondary embryonic mouse cultures. For optimal culturing of other cell types, a modification of 4500 mg/L glucose is available from MP

Dulbecco's Modification Of Eagle's Medium (1X Solution) Without L-Glutamine, Leucine, Sodium Pyruvate

Also known as DME, Dulbecco's Modified Eagle's Medium is the most widely used modification of Eagle's Basal Medium (BME). DMEM contains four times greater concentration of amino acids, vitamins and supplementary components. The original formulation calls for 1000 mg/L of glucose, which was first employed to support the polyoma virus in primary and secondary embryonic mouse cultures. For optimal culturing of other cell types, a modification of 4500 mg/L glucose is available from MP.

Dulbecco's Modification Of Eagle's Medium (DMEM) (1X Solution) Without L-Glutamine, Phenol Red

Also known as DME, Dulbecco's Modified Eagle's Medium is the most widely used modification of Eagle's Basal Medium (BME). DMEM contains four times greater concentration of amino acids, vitamins and supplementary components. The original formulation calls for 1000 mg/L of glucose, which was first employed to support the polyoma virus in primary and secondary embryonic mouse cultures. For optimal culturing of other cell types, a modification of 4500 mg/L glucose is available from MP.

Dulbecco's Modification Of Eagle's Medium (DMEM), Powder, With 4500 mg/L Dextrose, L-Glutamine, Without Sodium Bicarbonate, Sodium Pyruvate

Dulbecco's Modification of Eagle's Medium, with L-Glutamine, without Sodium Bicarbonate, without Sodium Pyruvate

Dulbecco's Modification Of Eagle's Medium With L-Glutamine and 4.5 g/L Glucose

Also known as DME, Dulbecco's Modified Eagle's Medium is the most widely used modification of Eagle's Basal Medium (BME). DMEM contains four times greater concentration of amino acids, vitamins and supplementary components. The original formulation calls for 1000 mg/L of glucose, which was first employed to support the polyoma virus in primary and secondary embryonic mouse cultures. For optimal culturing of other cell types, a modification of 4500 mg/L glucose is available from MP. S

Earle's Balanced Salts (10X Solution) Without Sodium Bicarbonate

Earle's Balanced Salts (10X Solution) without sodium bicarbonate

Earle's Balanced Salts (Modified) (EBS) (1X Solution) Without Phenol Red

Earle's Balanced Salts (Modified) (EBS) (1X Solution) without phenol red

Earle's Balanced Salts Without Sodium Bicarbonate

Earle's Balanced Salts without Sodium Bicarbonate. Particle Size: >90% thru 100 mesh pH (with 2200 mg/L NaHCO3): 7.57 Osmolality (with 2200 mg/L NaHCO3): 289 mM/kg Endotoxin: <0.10 EU/ml Mycoplasma: Negative

Minimum Essential Medium Eagle (Modified) (1X Solution) With Hank's Salts, 0.35 g/L Sodium Bicarbonate Without L-Glutamine

MEM, originally prepared by Harry Eagle, is one of the most popular cell culture media. Upon his attempts to cultivate normal mammalian fibroblasts and certain HeLa cell subtypes, it was revealed that the nutritional needs of these cell types could not be met by BME. Further studies led to the development of MEM incorporating specific modifications such as higher amino acid concentrations for the cultivation of fastidious cells. MP's MEM may be used to support the growth of cells in monolayers, in suspension and wide variety of other cell types with proper supplementation. MP offers MEM with either Earle's or Hanks' salts.

Minimum Essential Medium Eagle (Modified) (1X Solution) With Hank's Salts, 20 mM HEPES, Without L-Glutamine, Sodium Bicarbonate

MEM, originally prepared by Harry Eagle, is one of the most popular cell culture media. Upon his attempts to cultivate normal mammalian fibroblasts and certain HeLa cell subtypes, it was revealed that the nutritional needs of these cell types could not be met by BME. Further studies led to the development of MEM incorporating specific modifications such as higher amino acid concentrations for the cultivation of fastidious cells. MP's MEM may be used to support the growth of cells in monolayers, in suspension and wide variety of other cell types with proper supplementation. MP offers MEM with either Earle's or Hanks' salts.