Mammalian Media

67 Results Found

RPMI 1640 With 2 g/L Sodium Bicarbonate, Without L-Glutamine & Glucose

RPMI 1640 Medium was originally developed by Moore and his colleagues at Roswell Park Memorial Institute (RPMI). It was based on the RPMI 1630 line of media which utilized a bicarbonate buffering system and alterations in the amounts of amino acids and vitamins. A Dutch Modification of RPMI 1640 is also available through MP. The Dutch Modification contains 6400 mg/L sodium chloride instead of 6000 mg/L; contains 1000 mg/L sodium bicarbonate instead of 2000 mg/L; and contains 20 mM HEPES.

RPMI 1640 Without L-Glutamine

RPMI 1640 (1X Solution) without L-glutamine, pH 6.9 to 7.2. This medium was originally developed by Moore and his colleagues at Roswell Park Memorial Institute (RPMI). It was based on the RPMI 1630 line of media which utilized a bicarbonate buffering system and alterations in the amounts of amino acids and vitamins. RPMI has successfully been used for the cultivation of normal human and neoplastic leukocytes. It is now a popular general purpose medium when properly supplemented.

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100 mM Sodium Pyruvate (11.00 mg/mL)

Sodium pyruvate is used by cells as an easily accessible carbohydrate source. Additionally, it is involved with amino acid metabolism and initiates the Kreb′s cycle. The 100 mM solution should be diluted 1:100 for most cell culture. The use of sodium pyruvate in Wallen fermentation medium to enhance the conversion of oleic acid to 10-ketostearic acid by Bacillus sphaericus has been described. A protocol that uses sodium pyruvate to establish stably transfected human B cell lines has been published. It improves coliform recovery when present in culture medium.

Chicken Embryo Extract 20 mL

Chicken embryo extract is prepared from 9-12 day old eggs collected from registered flocks and found to be free from clinical signs of notifiable diseases.

Hat Supplement (50X Solution)

(50X Concentrate)Hypoxanthine 13.61 mg/LAminopterin 2H2O 0.1906 mg/LThymidine 3.876 mg/LSelective for hybridoma cell growth and growth of cells with a functioning HGPRT mechanism. The main application is for the selective growth of hybridoma cells for monoclonal antibody production.

HT (50X Solution)

(50X Concentrate) Hypoxanthine 13.61 mg/L Thymidine 3.876 mg/L This media is selective for growth of cells with a functioning HGPRT mechanism. The main application is for the selective growth of hybridoma cells for monoclonal antibody production.

ITS Premix Solution

ITS Premix will stimulate cell proliferation while decreasing substantially the serum requirements for culture of many cell types as diverse, for example, as contractile rat heart cells and human colon mucosal epithelial cells. Basal media supplemented with ITS Premix and as little as 2% Fetal Bovine Serum support proliferation of many diploid and heteroploid cell lines at rates equivalent to those obtained with 10% serum. Contains 0.5 mg/ml insulin from bovine pancreas, 0.5 mg/ml human transferrin (substantially iron-free) and 0.5 ug/ml sodium selenite. Prepared in Earle's balanced Salt Solution (EBSS) without phenol red. Sterile-filtered.

Williams Medium E, Powder, With L-Glutamine, Without Sodium Bicarbonate

Isolated epithelial cells, as described in his sequential plating method by Williams, et al., in 1971, were originally cultivated in a rich medium known as Williams' Medium D. From these early newborn animal studies, Williams and Gunn developed a subsequent medium, Williams' Medium E, for the long-term cultivation of adult rat liver epithelial cells.

High Growth Enhancement Medium, With L-Glutamine, Without Sodium Bicarbonate

High Growth Enhancement Medium is a modification of DMEM in which 3.6 g/liter fructose is substituted for glucose, resulting in better pH control and higher cell yields. Fructose is metabolized slower than glucose, resulting in slower acid buildup.

Ham's F-10 (10X Solution) Without L-Glutamine, Without Sodium Bicarbonate, With 12.4 mg/L Phenol Red

Originally, Ham's Nutrient Mixtures were developed for the clonal growth of several Chinese hamster ovary (CHO) clone cells, Hela cells and mouse L-cells. Both F-10 and F-12 are formulated for use with or without serum, depending on the type of cells being cultured. F-10 has demonstrated satisfactory growth of human diploid cells, white blood cells for chromosomal analysis and primary rat, rabbit and chicken tissue explants. Ham's F-12 is well suited for the growth of primary rat hepatocytes and prostate epithelial cells. It is also the medium of choice for toxicity assays using CHO cells. MP also offers Ham's F-12 supplemented with 25 mM HEPES for improved buffering in the optimum pH range of 7.2-7.4. F12K is a modification of Ham's F-12 and Coon's F-12 with increased amino acid and pyruvate concentrations. Additionally, the salts have been modifed (Konigsbergs). F12K has been developed for culturing differentiated rat and chicken cells, as well as, primary human liver cells.

Ham's F-10, With L-Glutamine, Without Sodium Bicarbonate

Originally, Ham's Nutrient Mixtures were developed for the clonal growth of several Chinese hamster ovary (CHO) clone cells, Hela cells and mouse L-cells. Both F-10 and F-12 are formulated for use with or without serum, depending on the type of cells being cultured. F-10 has demonstrated satisfactory growth of human diploid cells, white blood cells for chromosomal analysis and primary rat, rabbit and chicken tissue explants. Ham's F-12 is well suited for the growth of primary rat hepatocytes and prostate epithelial cells. It is also the medium of choice for toxicity assays using CHO cells. MP also offers Ham's F-12 supplemented with 25 mM HEPES for improved buffering in the optimum pH range of 7.2-7.4. F12K is a modification of Ham's F-12 and Coon's F-12 with increased amino acid and pyruvate concentrations. Additionally, the salts have been modifed (Konigsbergs). F12K has been developed for culturing differentiated rat and chicken cells, as well as, primary human liver cells.

Ham's F12 (1X Solution) With L-Glutamine

Originally, Ham's Nutrient Mixtures were developed for the clonal growth of several Chinese hamster ovary (CHO) clone cells, Hela cells and mouse L-cells. Both F-10 and F-12 are formulated for use with or without serum, depending on the type of cells being cultured. F-10 has demonstrated satisfactory growth of human diploid cells, white blood cells for chromosomal analysis and primary rat, rabbit and chicken tissue explants. Ham's F-12 is well suited for the growth of primary rat hepatocytes and prostate epithelial cells. It is also the medium of choice for toxicity assays using CHO cells. MP also offers Ham's F-12 supplemented with 25 mM HEPES for improved buffering in the optimum pH range of 7.2-7.4. F12K is a modification of Ham's F-12 and Coon's F-12 with increased amino acid and pyruvate concentrations. Additionally, the salts have been modifed (Konig

1X Hanks' Balanced Salt Solution (Modified) Without Phenol Red

Inorganic Salts: Calcium Chloride [CaCl2] 140.0000 mg/L, Magnesium Sulfate [MgSO4] 97.7000 mg/L, Potassium Chloride [KCl] 400.0000 mg/L, Potassium Phosphate Monobasic [KH2PO4] 60.0000 mg/L, Sodium Bicarbonate [NaHCO3] 350.0000 mg/L, Sodium Chloride [NaCl] 8000.0000 mg/L, Sodium Phosphate Dibasic [Na2HPO4] 47.7000 mg/L, Dextrose 1000.0000 mg/L

1X Hanks' Balanced Salt Solution, Modified

1× Hanks' Balanced Salt Solution, Modified

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1XHanks' Balanced Salts (Modified), Without Magnesium and Calcium

1× Hanks' Balanced Salts (Modified), without Magnesium and Calcium

Hank's Balanced Salts (Hbs) (Modified) (1X Solution) Without Calcium, Magnesium, Phenol Red

Hank's Balanced Salts (HBS) (Modified) (1X Solution) without calcium, magnesium, phenol red

High Gem (Growth Enhancement Medium) (1X Solution) Without L-Glutamine

High Growth Enhancement Medium, without L-Glutamine is a modification of DMEM in which 3.6 g/liter fructose is substituted for glucose, resulting in better pH control and higher cell yields. Fructose is metabolized slower than glucose, resulting in slower acid buildup.

Leibovitz L-15 Medium (Modified) (1X Solution) Without L-Glutamine

In this medium dextrose is replaced by galactose. In addition, buffering is provided by the free bases of the amino acids in place of sodium bicarbonate. As a result this medium has been used for growth of tissues in free gaseous exchange with the atmosphere.

1X Mccoy 5A Medium, Iwakata & Grace Modification With L-Glutamine

McCoy's 5A medium, as modified by Iwakata and Grace, is identical to RPMI 1629. Originally in 1959, McCoy and his colleagues described the amino acid requirements for the in vitro culturing of Novikoff Hepatoma cells. Basal Medium 5A was modified to create what is known as McCoy's 5A Medium for these investigations. Hsu and Kellogg further demonstrated the use of this medium to support the growth of primary cultures derived from normal bone marrow, testes, mouse kidney, skin, gingiva, rat embryo and other tissues. MP's medium is additionally suited for the propagation of leukocytes, biopsy tissues and the most demanding primary and continuous cell types. It is also available as modified by Park and Terasaki. The Park and Terasaki Modification (PT) differs from the Iwakata and Grace Modication (IG) in that PT contains slightly modified levels of L-glutamic acid, glycine, L-hydroxyproline, L-proline, L-serine, L-threonine, L-tryptophan, and L-valine. The PT modification also contains the additions of penicillin, dihydrostreptomycin sulfate, gentamycin, fetal bovine serum and HEPES.

Mccoy's 5A Medium (Park & Terasaki Modification) (1X Solution) Without L-Glutamine, Sodium Bicarbonate

McCoy's 5A medium, as modified by Iwakata and Grace, is identical to RPMI 1629. Originally in 1959, McCoy and his colleagues described the amino acid requirements for the in vitro culturing of Novikoff Hepatoma cells. Basal Medium 5A was modified to create what is known as McCoy's 5A Medium for these investigations. Hsu and Kellogg further demonstrated the use of this medium to support the growth of primary cultures derived from normal bone marrow, testes, mouse kidney, skin, gingiva, rat embryo and other tissues. MP's medium is additionally suited for the propagation of leukocytes, biopsy tissues and the most demanding primary and continuous cell types. It is also available as modified by Park and Terasaki.