Mammalian Media

67 Results Found

Medium 199 With Earle's Salt (E199), Powder, With L-Glutamine, Without Sodium Bicarbonate

Medium 199, originally described by Morgan and his colleagues (1950), is a completely defined nutritional source for cell culture. Their investigations demonstrated that cell growth could be measured in this medium. It has broad species applicability including the culturing of non-transformed cell types. It may be used for vaccine production and the in vitro cultivation of rat lens tissues and primary mouse pancreatic epithelial explants. It is available in either Earle's or Hanks' salts.

Medium 199 With Hanks' Salts (H199), Powder, With L-Glutamine Without Sodium Bicarbonate

Medium 199, originally described by Morgan and his colleagues (1950), is a completely defined nutritional source for cell culture. Their investigations demonstrated that cell growth could be measured in this medium. It has broad species applicability including the culturing of non-transformed cell types. It may be used for vaccine production and the in vitro cultivation of rat lens tissues and primary mouse pancreatic epithelial explants. It is available in either Earle's or Hanks' salts.

Dulbecco's Modification Of Eagle's Medium (DMEM), Powder, With 4500 mg/L Dextrose, L-Glutamine, Without Sodium Bicarbonate

Dulbecco's Modified Eagle's Medium (DMEM) is the most widely used modification of Eagle's Basal Medium (BME). DMEM contains four times greater concentration of amino acids, vitamins and supplementary components. The original formulation calls for 1000 mg/L of glucose, which was first employed to support the polyoma virus in primary and secondary embryonic mouse cultures. For optimal culturing of other cell types, a modification of 4500 mg/L glucose is available.

Biorich W/Gln

BioRich 1 is a DMEM/F-12 powdered media blend enriched with trace elements. This permits the transition to "serum-free" conditions reducing or eliminating the necessity for serum supplementation. It is used with ITS Premix, a completely serum independent.

RPMI 1640, With L-Glutamine, Without Sodium Bicarbonate

This medium was originally developed by Moore and his colleagues at Roswell Park Memorial Institute (RPMI). It was based on the RPMI 1630 line of media which utilized a bicarbonate buffering system and alterations in the amounts of amino acids and vitamins. RPMI has successfully been used for the cultivation of normal human and neoplastic leukocytes. It is now a popular general purpose medium when properly supplemented.

Minimum Essential Medium Eagle (Modified) With Earle's Salts Without L-Glutamine Or Sodium Bicarbonate

MEM, originally prepared by Harry Eagle, is one of the most popular cell culture media. Upon his attempts to cultivate normal mammalian fibroblasts and certain HeLa cell subtypes, it was revealed that the nutritional needs of these cell types could not be met by BME. Further studies led to the development of MEM incorporating specific modifications such as higher amino acid concentrations for the cultivation of fastidious cells. MP's MEM may be used to support the growth of cells in monolayers, in suspension and wide variety of other cell types with proper supplementation. MP offers MEM with either Earle's or Hanks' salts.

Minimum Essential Medium Eagle (Modified) (1X Solution) Without L-Glutamine

MEM, originally prepared by Harry Eagle, is one of the most popular cell culture media. Upon his attempts to cultivate normal mammalian fibroblasts and certain HeLa cell subtypes, it was revealed that the nutritional needs of these cell types could not be met by BME. Further studies led to the development of MEM incorporating specific modifications such as higher amino acid concentrations for the cultivation of fastidious cells. MP's MEM may be used to support the growth of cells in monolayers, in suspension and wide variety of other cell types with proper supplementation. MP offers MEM with either Earle's or Hanks' salts.

Dulbecco's Modified Eagle's Medium (DMEM) (1X Solution) With 20 mM HEPES, Without L-Glutamine Or Sodium Bicarbonate

Also known as DME, Dulbecco's Modified Eagle's Medium is the most widely used modification of Eagle's Basal Medium (BME). DMEM contains four times greater concentration of amino acids, vitamins and supplementary components. The original formulation calls for 1000 mg/L of glucose, which was first employed to support the polyoma virus in primary and secondary embryonic mouse cultures. For optimal culturing of other cell types, a modification of 4500 mg/L glucose is available from MP.

RPMI 1640 (1X Solution) With L-Glutamine With Sodium Bicarbonate

RPMI 1640 Medium was originally developed by Moore and his colleagues at Roswell Park Memorial Institute (RPMI). It was based on the RPMI 1630 line of media which utilized a bicarbonate buffering system and alterations in the amounts of amino acids and vitamins. A Dutch Modification of RPMI 1640 is also available through MP. The Dutch Modification contains 6400 mg/L sodium chloride instead of 6000 mg/L, 1000 mg/L sodium bicarbonate instead of 2000 mg/L, and 20 mM HEPES.

Phosphate Buffered Saline (PBS) (1X Solution) Dulbecco's Formula Without Calcium, Magnesium

Phosphate Buffered Salts, Dulbecco's Formula (DPBS) is a balanced salt solution (BSS) used for the handling and culturing of mammalian cells. DPBS is used to irrigate, wash, and dilute mammalian cells. Phosphate buffering maintains the pH in the physiological range. Calcium and magnesium facilitate cell binding and clumping. DPBS without these ions can be used to wash and rinse suspended cells.

CELLect Fetal Bovine Serum, Silver, Mexico Origin

CELLect™ Silver is collected from a U.S.D.A. regulated and monitored abbatoir by technicians who work "on-site" where the fetal blood is aseptically collected via cardiac puncture. This raw material is transported to the production facility where it undergoes sterilization via filtration with multiple 0.1 micron filters. The care and diligence taken in handling the fetal serum minimizes cell lysis and other detrimental factors thereby reducing possible lysozyme and hemoglobin contamination. This translates into increased performance in the final product. It is supplied sterile, with no detectable mycoplasma or viral infection.

CELLect Fetal Bovine Serum, Gold, Us Origin

CELLect™ Gold is collected from a U.S.D.A. regulated and monitored abattoir by technicians who work "on-site" where the fetal blood is aseptically collected via cardiac puncture. This raw material is transported to the production facility where it undergoes sterilization via filtration through multiple 0.1 micron filters. The care and diligence taken in handling the fetal serum minimizes cell lysis and other detrimental factors thereby reducing possible lysozyme and hemoglobin contamination. This translates into increased performance in the final product. It is superior for hybridoma cloning. It is supplied sterile, with no detectable mycoplasma or viral infection. Serum is an extremely complex mixture of many small and large biomolecules with different, physiologically balanced growth-promoting and growth inhibiting activities.

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CELLect Fetal Bovine Serum, Gold, Heat Inactivated

Fetal bovine serum has been the most widely used growth supplement for cell culture media because of its high content of embryonic growth-promoting factors. When used at appropriate concentrations, it supplies many defined and undefined components that have been shown to satisfy specific metabolic requirements for the culture of cells in vitro.

Minimum Essential Medium Eagle (Modified), With Earle's Salts and L-Glutamine, Without Sodium Bicarbonate

Minimum Essential Medium Eagle (Modified), with Earle's Salts and L-Glutamine, without Sodium Bicarbonate was originally prepared by Harry Eagle and is one of the most popular cell culture media. The formulation differs from the original in that the concentrations of some of the vitamins have been increased slightly; in some, the amino acids differ by decimal amounts. In some formulation, the MgSO4 concentration differs from that of Eagle but agrees with that quoted by Parker (Parker quotes 200.00 mg/liter MgSO4.7H2O).

1X RPMI Without L-Glutamine, L-Cysteine, L-Cystine, and L-Methionine

RPMI 1640 Medium was originally developed by Moore and his colleagues at Roswell Park Memorial Institute (RPMI). It was based on the RPMI 1630 line of media which utilized a bicarbonate buffering system and alterations in the amounts of amino acids and vitamins. A Dutch Modification of RPMI 1640 is also available through MP. The Dutch Modification contains 6400 mg/L sodium chloride instead of 6000 mg/L; contains 1000 mg/L sodium bicarbonate instead of 2000 mg/L; and contains 20 mM HEPES.

1X RPMI 1640 Without L-Glutamine and Phosphate, With 0.85 g/L Sodium Bicarbonate

RPMI 1640 Medium was originally developed by Moore and his colleagues at Roswell Park Memorial Institute (RPMI). It was based on the RPMI 1630 line of media which utilized a bicarbonate buffering system and alterations in the amounts of amino acids and vitamins. A Dutch Modification of RPMI 1640 is also available through MP. The Dutch Modification contains 6400 mg/L sodium chloride instead of 6000 mg/L, 1000 mg/L sodium bicarbonate instead of 2000 mg/L, and 20 mM HEPES.

1X RPMI 1640 Without L-Glutamine, Phenol Red

This medium was originally developed by Moore and his colleagues at Roswell Park Memorial Institute (RPMI). It was based on the RPMI 1630 line of media which utilized a bicarbonate buffering system and alterations in the amounts of amino acids and vitamins. RPMI has successfully been used for the cultivation of normal human and neoplastic leukocytes. It is now a popular general purpose medium when properly supplemented. Without L-glutamine, phenol red; Endotoxin Tested; Sterile Filtered

RPMI 1640 (1X Solution) Without L-Glutamine and L-Leucine

RPMI 1640 was originally developed by Moore and his colleagues at Roswell Park Memorial Institute (RPMI). It was based on the RPMI 1630 line of media which utilized a bicarbonate buffering system and alterations in the amounts of amino acids and vitamins. A Dutch Modification of RPMI 1640 is also available through MP. The Dutch Modification contains 6400 mg/L sodium chloride instead of 6000 mg/L, 1000 mg/L sodium bicarbonate instead of 2000 mg/L, and 20 mM HEPES.

RPMI 1640 With 2 g/L Sodium Bicarbonate, Without L-Glutamine & Glucose

RPMI 1640 Medium was originally developed by Moore and his colleagues at Roswell Park Memorial Institute (RPMI). It was based on the RPMI 1630 line of media which utilized a bicarbonate buffering system and alterations in the amounts of amino acids and vitamins. A Dutch Modification of RPMI 1640 is also available through MP. The Dutch Modification contains 6400 mg/L sodium chloride instead of 6000 mg/L; contains 1000 mg/L sodium bicarbonate instead of 2000 mg/L; and contains 20 mM HEPES.