Casein Hydrolysate Enzymatic
Casein hydrolysate enzymatic is a pancreatic hydrolysate of casein. This hydrolysate contains, in the form of mixed amino acids and peptides, all amino acids originally present in casein. It is a refined product providing high quality soluble source of amino acids,polypeptides and peptides.
Murashige and Skoog Medium, Without Sucrose, Agar, Iaa, and Kinetin
Murashige and Skoog Medium contains Murashige and Skoog salts, as well as a mixture of vitamins. This modification of the original formula does not contain agar, sucrose, IAA, and Kinetin. Murashige and Skoog Medium is widely used plant tissue culture growth medium. It has proven effective in the culture of tissue derived from moncotyledons and dicotyledons. This medium, originally formulated to support tobacco callus, has been used to support callus initiation and growth, the growth of cells in suspension culture and the regeneration of shoots and plantlets from explants. More recently, it has been used for micropropagation of ornamental, vegetable and fruit plants in research and commercial enterprises.
Alpha Modification Of Eagle's Medium (AMEM), Powder, With L-Glutamine, Without Deoxyribosides, Ribosides, Sodium Bicarbonate
Alpha Modification of Eagle's Medium (AMEM), powder, with L-glutamine, without deoxyribosides, ribosides, sodium bicarbonate. Originally used for growth of hamster kidney cells.
Ham's F-12 (Modified) With L-Glutamine, Without Sodium Bicarbonate
Originally, Ham's Nutrient Mixtures were developed for the clonal growth of several Chinese hamster ovary (CHO) clone cells, Hela cells and mouse L-cells. Both F-10 and F-12 are formulated for use with or without serum, depending on the type of cells being cultured. F-10 has demonstrated satisfactory growth of human diploid cells, white blood cells for chromosomal analysis and primary rat, rabbit and chicken tissue explants. Ham's F-12 is well suited for the growth of primary rat hepatocytes and prostate epithelial cells. It is also the medium of choice for toxicity assays using CHO cells. MP also offers Ham's F-12 supplemented with 25 mM HEPES for improved buffering in the optimum pH range of 7.2-7.4. F12K is a modification of Ham's F-12 and Coon's F-12 with increased amino acid and pyruvate concentrations. Additionally, the salts have been modifed (Konigsbergs). F12K has been developed for culturing differentiated rat and chicken cells, as well as, primary human liver cells. MgCl2 has been substituted by MgSO4 containing an equivalent amount of Mg ion.
Hanks' Balanced Salts, Powder, Without Sodium Bicarbonate
Hanks' Balanced Salts solution is used to maintain the pH and osmotic balance in the medium and to provide the cells with water and essential inorganic ions.
Phosphate Buffered Saline (PBS), Dulbecco's Formula, Powder, Without Calcium, Magnesium
Phosphate Buffered Salts, Dulbecco's Formula (DPBS) is a balanced salt solution (BSS) used for the handling and culturing of mammalian cells. DPBS is used to irrigate, wash, and dilute mammalian cells. Phosphate buffering maintains the pH in the physiological range. Calcium and magnesium facilitate cell binding and clumping. DPBS without these ions can be used to wash and rinse suspended cells.