Bacterial Media

170 Results Found

10X Dulbecco's Phosphate Buffered Saline, Without Calcium and Magnesium

Role of a balanced salt solution in cell culture is multifaceted and can be divided into four principal functions: 1. Serves as an irrigating, transporting and diluting fluid while maintaining intra- and extracellular osmotic balance; 2. Provides cells with water and certain bulk inorganic ions essential for normal cell metabolism; 3. Combined with a carbohydrate, such as glucose, provides the principal energy source for cell metabolism; 4. Provides a buffering system to maintain the medium within the physiological pH range (7.2 - 7.6)

Murashige and Skoog's Modified Vitamin Solution (1000X)

Includes the vitamins as described by Murashige and Skoog, 1962. Use at a concentration of 1 ml/L medium.

Murashige and Skoog Basal Salt Medium With Gamborg's Vitamins

Includes the macro- and mironutrients as described by Murashige and Skoog, 1962, and vitamins as described by Gamborg, et al., 1966.

Minimum Essential Medium Eagle (Modified) With Earle's Salts, L-Glutamine, Non-Essential Amino Acids, Without Sodium Bicarbonate

(MEM Powder, Modified)
With Earle's salts, non-essential amino acids, and L-glutamine
Without sodium bicarbonate

Minimal Essential Medium Non-Essential Amino Acids (100X Solution)

100X Non-essential Amino Acids for Minimum Essential Medium Eagle Contains 19 amino acids. The essential amino acids and the non-essential amino acids; L-ala; L-asn; L-asp; L-glu; L-gly; L-pro and L-ser.

MEM Amino Acids (50X Solution) Without L-Glutamine

In many cell culture applications using defined media for the in vitro cultivation of cells, the addition of supplemental nutrients and reagents is required. Additives such as antibiotics, buffers, and stains are frequently used to prevent bacterial contamination, control pH, and visibly monitor media conditions. Other supplements such as amino acids and vitamins are useful for enriched media beyond their normal concentrations. Attachment factors and transport factors are added to growth media to facilitate rapid cell propagation and enhance proper cell metabolism. Still other media supplements may be added to create conditions which will elicit specific cellular responses and effects, such as altered protein biosynthesis, accelerated antibody production and secretion, toxic response mechanisms, etc. As a result, the availability of a wide variety of media supplements is desirable when designing cell culture experiments.

Mccoy's 5A Medium (Park & Terasaki Modification) (1X Solution) Without L-Glutamine, Sodium Bicarbonate

McCoy's 5A medium, as modified by Iwakata and Grace, is identical to RPMI 1629. Originally in 1959, McCoy and his colleagues described the amino acid requirements for the in vitro culturing of Novikoff Hepatoma cells. Basal Medium 5A was modified to create what is known as McCoy's 5A Medium for these investigations. Hsu and Kellogg further demonstrated the use of this medium to support the growth of primary cultures derived from normal bone marrow, testes, mouse kidney, skin, gingiva, rat embryo and other tissues. MP's medium is additionally suited for the propagation of leukocytes, biopsy tissues and the most demanding primary and continuous cell types. It is also available as modified by Park and Terasaki.

1X Mccoy 5A Medium, Iwakata & Grace Modification With L-Glutamine

McCoy's 5A medium, as modified by Iwakata and Grace, is identical to RPMI 1629. Originally in 1959, McCoy and his colleagues described the amino acid requirements for the in vitro culturing of Novikoff Hepatoma cells. Basal Medium 5A was modified to create what is known as McCoy's 5A Medium for these investigations. Hsu and Kellogg further demonstrated the use of this medium to support the growth of primary cultures derived from normal bone marrow, testes, mouse kidney, skin, gingiva, rat embryo and other tissues. MP's medium is additionally suited for the propagation of leukocytes, biopsy tissues and the most demanding primary and continuous cell types. It is also available as modified by Park and Terasaki. The Park and Terasaki Modification (PT) differs from the Iwakata and Grace Modication (IG) in that PT contains slightly modified levels of L-glutamic acid, glycine, L-hydroxyproline, L-proline, L-serine, L-threonine, L-tryptophan, and L-valine. The PT modification also contains the additions of penicillin, dihydrostreptomycin sulfate, gentamycin, fetal bovine serum and HEPES.

Leibovitz L-15 Medium (Modified) (1X Solution) Without L-Glutamine

In this medium dextrose is replaced by galactose. In addition, buffering is provided by the free bases of the amino acids in place of sodium bicarbonate. As a result this medium has been used for growth of tissues in free gaseous exchange with the atmosphere.

High Gem (Growth Enhancement Medium) (1X Solution) Without L-Glutamine

High Growth Enhancement Medium, without L-Glutamine is a modification of DMEM in which 3.6 g/liter fructose is substituted for glucose, resulting in better pH control and higher cell yields. Fructose is metabolized slower than glucose, resulting in slower acid buildup.

Hank's Balanced Salts (Hbs) (Modified) (1X Solution) Without Calcium, Magnesium, Phenol Red

Hank's Balanced Salts (HBS) (Modified) (1X Solution) without calcium, magnesium, phenol red

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1XHanks' Balanced Salts (Modified), Without Magnesium and Calcium

1× Hanks' Balanced Salts (Modified), without Magnesium and Calcium

1X Hanks' Balanced Salt Solution, Modified

1× Hanks' Balanced Salt Solution, Modified

1X Hanks' Balanced Salt Solution (Modified) Without Phenol Red

Inorganic Salts: Calcium Chloride [CaCl2] 140.0000 mg/L, Magnesium Sulfate [MgSO4] 97.7000 mg/L, Potassium Chloride [KCl] 400.0000 mg/L, Potassium Phosphate Monobasic [KH2PO4] 60.0000 mg/L, Sodium Bicarbonate [NaHCO3] 350.0000 mg/L, Sodium Chloride [NaCl] 8000.0000 mg/L, Sodium Phosphate Dibasic [Na2HPO4] 47.7000 mg/L, Dextrose 1000.0000 mg/L

Ham's F12 (1X Solution) With L-Glutamine

Originally, Ham's Nutrient Mixtures were developed for the clonal growth of several Chinese hamster ovary (CHO) clone cells, Hela cells and mouse L-cells. Both F-10 and F-12 are formulated for use with or without serum, depending on the type of cells being cultured. F-10 has demonstrated satisfactory growth of human diploid cells, white blood cells for chromosomal analysis and primary rat, rabbit and chicken tissue explants. Ham's F-12 is well suited for the growth of primary rat hepatocytes and prostate epithelial cells. It is also the medium of choice for toxicity assays using CHO cells. MP also offers Ham's F-12 supplemented with 25 mM HEPES for improved buffering in the optimum pH range of 7.2-7.4. F12K is a modification of Ham's F-12 and Coon's F-12 with increased amino acid and pyruvate concentrations. Additionally, the salts have been modifed (Konig

Ham's F-10, With L-Glutamine, Without Sodium Bicarbonate

Originally, Ham's Nutrient Mixtures were developed for the clonal growth of several Chinese hamster ovary (CHO) clone cells, Hela cells and mouse L-cells. Both F-10 and F-12 are formulated for use with or without serum, depending on the type of cells being cultured. F-10 has demonstrated satisfactory growth of human diploid cells, white blood cells for chromosomal analysis and primary rat, rabbit and chicken tissue explants. Ham's F-12 is well suited for the growth of primary rat hepatocytes and prostate epithelial cells. It is also the medium of choice for toxicity assays using CHO cells. MP also offers Ham's F-12 supplemented with 25 mM HEPES for improved buffering in the optimum pH range of 7.2-7.4. F12K is a modification of Ham's F-12 and Coon's F-12 with increased amino acid and pyruvate concentrations. Additionally, the salts have been modifed (Konigsbergs). F12K has been developed for culturing differentiated rat and chicken cells, as well as, primary human liver cells.

Ham's F-10 (10X Solution) Without L-Glutamine, Without Sodium Bicarbonate, With 12.4 mg/L Phenol Red

Originally, Ham's Nutrient Mixtures were developed for the clonal growth of several Chinese hamster ovary (CHO) clone cells, Hela cells and mouse L-cells. Both F-10 and F-12 are formulated for use with or without serum, depending on the type of cells being cultured. F-10 has demonstrated satisfactory growth of human diploid cells, white blood cells for chromosomal analysis and primary rat, rabbit and chicken tissue explants. Ham's F-12 is well suited for the growth of primary rat hepatocytes and prostate epithelial cells. It is also the medium of choice for toxicity assays using CHO cells. MP also offers Ham's F-12 supplemented with 25 mM HEPES for improved buffering in the optimum pH range of 7.2-7.4. F12K is a modification of Ham's F-12 and Coon's F-12 with increased amino acid and pyruvate concentrations. Additionally, the salts have been modifed (Konigsbergs). F12K has been developed for culturing differentiated rat and chicken cells, as well as, primary human liver cells.

High Growth Enhancement Medium, With L-Glutamine, Without Sodium Bicarbonate

High Growth Enhancement Medium is a modification of DMEM in which 3.6 g/liter fructose is substituted for glucose, resulting in better pH control and higher cell yields. Fructose is metabolized slower than glucose, resulting in slower acid buildup.

Minimum Essential Medium Eagle With Earle's Salts and 2.0 g/L Sodium Bicarbonate Without L-Glutamine, L-Cysteine, L-Cystine, and L-Methionine

MEM, originally prepared by Harry Eagle, is one of the most popular cell culture media. Upon his attempts to cultivate normal mammalian fibroblasts and certain HeLa cell subtypes, it was revealed that the nutritional needs of these cell types could not be met by BME. Further studies led to the development of MEM incorporating specific modifications such as higher amino acid concentrations for the cultivation of fastidious cells. MP's MEM may be used to support the growth of cells in monolayers, in suspension and wide variety of other cell types with proper supplementation. MP offers MEM with either Earle's or Hanks' salts.