Bacterial Media

170 Results Found

Supplement VX

Supplement VX is recommended as an enrichment for the cultivation of Neisseria gonorrhoeae, Haemophilus influenzae and other fastidious organisms. Supplement VX is a sterile lyophilized concentrate of essential growth factors for supplementing media for culturing microorganisms with exacting growth requirements. It is used for the preparation of chocolate agar from GC Agar Base and hemoglobin for isolation and cultivation of N. gonorrhoeae. It is also recommended for enriching Brain Heart Infustion Agar and Brain Heart Infusion Broth

ITS Premix Solution

ITS Premix will stimulate cell proliferation while decreasing substantially the serum requirements for culture of many cell types as diverse, for example, as contractile rat heart cells and human colon mucosal epithelial cells. Basal media supplemented with ITS Premix and as little as 2% Fetal Bovine Serum support proliferation of many diploid and heteroploid cell lines at rates equivalent to those obtained with 10% serum. Contains 0.5 mg/ml insulin from bovine pancreas, 0.5 mg/ml human transferrin (substantially iron-free) and 0.5 ug/ml sodium selenite. Prepared in Earle's balanced Salt Solution (EBSS) without phenol red. Sterile-filtered.

HT (50X Solution)

(50X Concentrate) Hypoxanthine 13.61 mg/L Thymidine 3.876 mg/L This media is selective for growth of cells with a functioning HGPRT mechanism. The main application is for the selective growth of hybridoma cells for monoclonal antibody production.

Hat Supplement (50X Solution)

(50X Concentrate)Hypoxanthine 13.61 mg/LAminopterin 2H2O 0.1906 mg/LThymidine 3.876 mg/LSelective for hybridoma cell growth and growth of cells with a functioning HGPRT mechanism. The main application is for the selective growth of hybridoma cells for monoclonal antibody production.

Chicken Embryo Extract 20 mL

Chicken embryo extract is prepared from 9-12 day old eggs collected from registered flocks and found to be free from clinical signs of notifiable diseases.

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100 mM Sodium Pyruvate (11.00 mg/mL)

Sodium pyruvate is used by cells as an easily accessible carbohydrate source. Additionally, it is involved with amino acid metabolism and initiates the Kreb′s cycle. The 100 mM solution should be diluted 1:100 for most cell culture. The use of sodium pyruvate in Wallen fermentation medium to enhance the conversion of oleic acid to 10-ketostearic acid by Bacillus sphaericus has been described. A protocol that uses sodium pyruvate to establish stably transfected human B cell lines has been published. It improves coliform recovery when present in culture medium.

TCM® Defined Serum Replacement

TCM™ is a fortified, multipurpose serum replacement for long-term culturing of many types of anchorage dependent and suspension cultures. TCM® Defined Serum Replacement shows following features: A universal serum replacement that supports human and mammalian cells; completely free of virus, mycoplasma and other infectious agents; contains no growth factors, steroid hormones, extraneous proteins or phenol red; protein origin - human; Drug Master on file with the FDA.

TCH® Defined Serum Replacement, 50× Concentrate

TCH is a fortified, multipurpose serum replacement for long-term culturing of primarily human anchorage-dependent and suspension cells. TCH® Defined Serum Replacement shows following features: A universal serum replacement that supports human and mammalian cells; completely free of virus, mycoplasma and other infectious agents; contains no growth factors, steroid hormones, extraneous proteins or phenol red; protein origin - human; Drug Master on file with the FDA.

Mp-Mrc-5 Serum-Free Cell Culture Medium

MP-MRC-5® Medium, Serum Free, very low protein (90 ng/mL) medium developed specifically for the culture of MRC-5 cells. MP-MRC-5™ medium was developed specifically for microcarrier culture of MRC-5 cells in production systems. It is completely animal component-free and contains very little protein. MP-MRC-5™ achieves growth rates superior to standard serum-supplemented media. Adaptation from serum-supplemented media is not necessary.

Sabouraud Dextrose Agar Without Chloramphenicol

Sabouraud Dextrose Agar can be used for the isolation, identification and maintainance of pathogenic and saprophytic fungi. It is suited for the cultivation of yeasts, molds and aciduric microorganisms.

RPMI 1640 Without L-Glutamine

RPMI 1640 (1X Solution) without L-glutamine, pH 6.9 to 7.2. This medium was originally developed by Moore and his colleagues at Roswell Park Memorial Institute (RPMI). It was based on the RPMI 1630 line of media which utilized a bicarbonate buffering system and alterations in the amounts of amino acids and vitamins. RPMI has successfully been used for the cultivation of normal human and neoplastic leukocytes. It is now a popular general purpose medium when properly supplemented.

RPMI 1640 With 2 g/L Sodium Bicarbonate, Without L-Glutamine & Glucose

RPMI 1640 Medium was originally developed by Moore and his colleagues at Roswell Park Memorial Institute (RPMI). It was based on the RPMI 1630 line of media which utilized a bicarbonate buffering system and alterations in the amounts of amino acids and vitamins. A Dutch Modification of RPMI 1640 is also available through MP. The Dutch Modification contains 6400 mg/L sodium chloride instead of 6000 mg/L; contains 1000 mg/L sodium bicarbonate instead of 2000 mg/L; and contains 20 mM HEPES.

RPMI 1640 (1X Solution) Without L-Glutamine and L-Leucine

RPMI 1640 was originally developed by Moore and his colleagues at Roswell Park Memorial Institute (RPMI). It was based on the RPMI 1630 line of media which utilized a bicarbonate buffering system and alterations in the amounts of amino acids and vitamins. A Dutch Modification of RPMI 1640 is also available through MP. The Dutch Modification contains 6400 mg/L sodium chloride instead of 6000 mg/L, 1000 mg/L sodium bicarbonate instead of 2000 mg/L, and 20 mM HEPES.

1X RPMI 1640 Without L-Glutamine, Phenol Red

This medium was originally developed by Moore and his colleagues at Roswell Park Memorial Institute (RPMI). It was based on the RPMI 1630 line of media which utilized a bicarbonate buffering system and alterations in the amounts of amino acids and vitamins. RPMI has successfully been used for the cultivation of normal human and neoplastic leukocytes. It is now a popular general purpose medium when properly supplemented. Without L-glutamine, phenol red; Endotoxin Tested; Sterile Filtered

1X RPMI 1640 Without L-Glutamine and Phosphate, With 0.85 g/L Sodium Bicarbonate

RPMI 1640 Medium was originally developed by Moore and his colleagues at Roswell Park Memorial Institute (RPMI). It was based on the RPMI 1630 line of media which utilized a bicarbonate buffering system and alterations in the amounts of amino acids and vitamins. A Dutch Modification of RPMI 1640 is also available through MP. The Dutch Modification contains 6400 mg/L sodium chloride instead of 6000 mg/L, 1000 mg/L sodium bicarbonate instead of 2000 mg/L, and 20 mM HEPES.

1X RPMI Without L-Glutamine, L-Cysteine, L-Cystine, and L-Methionine

RPMI 1640 Medium was originally developed by Moore and his colleagues at Roswell Park Memorial Institute (RPMI). It was based on the RPMI 1630 line of media which utilized a bicarbonate buffering system and alterations in the amounts of amino acids and vitamins. A Dutch Modification of RPMI 1640 is also available through MP. The Dutch Modification contains 6400 mg/L sodium chloride instead of 6000 mg/L; contains 1000 mg/L sodium bicarbonate instead of 2000 mg/L; and contains 20 mM HEPES.

Pseudomonas Agar F

Used with glycerol (cat. no. 1004417) for the isolation, cultivation and differentiation of Pseudomonas aeruginosa on the basis of phycocyanin (pigment) production.

Potato Dextrose Broth

Potato Dextrose Broth is used for the isolation, enumeration and identification of yeast and molds. It can be used in the analysis of dairy products, bottled drinks, frozen food and other types of food. It can also be used in the identification of fungi and yeasts in parallel with their cellular morphology or in methods of mirocultivation in slides. When the medium is to be used for enumeration of molds and yeasts, add to the medium, sterilized and cooled to 45-50 °C, approximately 14 mL of a sterilized 10% solution of tartaric acid to obtain a pH of 3.5.

Pluronic® F-68 Polyol, 10% Solution, (100×)

Pluronic F-68® is a difunctional block copolymer surfactant terminating in primary hydroxyl groups. It is a non-ionic detergent that protects cells from hydrodynamic damage. It can be also used for in vitro isolation of lymphocytes from diluted whole blood.

Pluronic® F-68 Polyol

Pluronic F-68® is a difunctional block copolymer surfactant terminating in primary hydroxyl groups. It is a non-ionic detergent that protects cells from hydrodynamic damage. It can be also used for in vitro isolation of lymphocytes from diluted whole blood.